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Antimicrobial activity, cytotoxic effect and characterization of marine bivalve extracts Cerastoderma glaucum
Rendiconti Lincei. Scienze Fisiche e Naturali ( IF 1.810 ) Pub Date : 2020-10-31 , DOI: 10.1007/s12210-020-00964-0
Mohamed H. Mona , Gihan M. El-Khodary , Nahla E. Omran , Karolin K. Abd El-Aziz , Salwa A. El-Saidy

Cerastoderma glaucum, a marine bivalve inhabiting Lake Timsah, is surrounded by different pathogenic organisms. The present study evaluates the antimicrobial activities, cytotoxicity and characterization of C. glaucum extracts. Chloroform, methanol and acidic tissue extracts were prepared from C. glaucum collected during winter and summer seasons. Winter acidic extract exhibits potent antimicrobial activities against 21 bacterial, 2 yeast and 2 viral strains. The inhibition zone of this extract ranges from 10 mm against Klebsiella oxytoca, Pseudomonas stutzeri, Globicatella sulfidifaciens and Bacillus (B. badius, B. amyloliquefaciens and B. pumilus) to 24 mm against Shigella flexneri. Also, the inhibition viral activities of this extract at a concentration of 62.5 µg ml−1 against Hepatitis A virus and Herpes simplex virus type 1 (HSV-1) are 62.383% and 57.035%, respectively, with low cytotoxicity of 24.030%. Furthermore, winter acidic extract of C. glaucum has the highest total protein contents (9.8 mg ml−1) compared with the other extracts. Moreover, the sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) indicates the presence of four clear low molecular weight peptides bands; 8.588, 7.237, 4.423 and 2.692 kDa. Fourier transform-infrared (FT-IR) analysis indicates the presence of 12 functional groups of proteins in winter acidic extract of C. glaucum at appropriate wavelengths.



中文翻译:

海洋双壳类植物提取物青霉菌的抗菌活性,细胞毒性作用和表征

青草皮,生活在蒂姆萨湖(Lake Timsah)的海洋双壳类动物,被不同的病原生物所包围。本研究评估了青衣提取物的抗菌活性,细胞毒性和表征。从冬季和夏季采集的青柠条制备氯仿,甲醇和酸性组织提取物。冬季酸性提取物对21种细菌,2种酵母和2种病毒株表现出有效的抗菌活性。从对10mM该提取物范围的抑制区产酸克雷伯菌,施氏假单胞菌,Globicatella sulfidifaciens芽孢杆菌B.栗褐解淀粉芽孢杆菌短小芽孢杆菌)对抗志贺志贺菌24毫米。另外,该提取物中的62.5微克ml的浓度抑制病毒活性-1针对A型肝炎病毒和单纯疱疹病毒1型(HSV-1)分别是62.383%和57.035%,具有24.030%的低细胞毒性。此外,青柠檬的冬季酸性提取物具有最高的总蛋白质含量(9.8 mg ml -1)与其他提取物相比。此外,十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)指示存在四个清晰的低分子量肽带。8.588、7.237、4.423和2.692 kDa。傅里叶变换红外(FT-IR)分析表明,在适当的波长下,青柠豆冬季酸性提取物中存在12个官能团的蛋白质。

更新日期:2020-11-02
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