Titanium and titanium alloys are widely used as a biomaterial due to their mechanical strength, corrosion resistance, low elastic modulus, and excellent biocompatibility. TiO₂ nanotubes have excellent bioactivity, stimulating the adhesion, proliferation of fibroblasts and adipose-derived stem cells, production of alkaline phosphatase by osteoblasts, platelets activation, growth of neural cells and adhesion, spreading, growth, and differentiation of rat bone marrow mesenchymal stem cells. In this study, we investigated the functionality of fibroblast on titania nanotube layers annealed at different temperatures. The titania nanotube layer was fabricated by potentiostatic anodization of titanium, then annealed at 300, 530, and 630 °C for 5 h. The resulting nanotube layer was characterized using SEM (Scanning Electron Microscopy), TF-XRD (Thin-film X-ray diffraction), and contact angle goniometry. Fibroblasts viability was determined by the CellTiter-Blue method and cytotoxicity by Lactate Dehydrogenase test, and the cell morphology was analyzed by scanning electron microscopy. Also, cell adherence, proliferation, and morphology were analyzed by fluorescence microscopy. The results indicate that the modification in nanotube crystallinity may provide a favorable surface fibroblast growth, especially on substrates annealed at 530 and 630 °C, indicating that these properties provide a favorable template for biomedical implants.

钛及钛合金由于其机械强度高、耐腐蚀、弹性模量低和良好的生物相容性而被广泛用作生物材料。二氧化钛₂纳米管具有优异的生物活性，刺激成纤维细胞和脂肪干细胞的粘附、增殖，成骨细胞产生碱性磷酸酶，激活血小板，促进神经细胞的生长以及大鼠骨髓间充质干细胞的粘附、扩散、生长和分化。在这项研究中，我们研究了成纤维细胞在不同温度下退火的二氧化钛纳米管层上的功能。二氧化钛纳米管层是通过钛的恒电位阳极氧化制造的，然后在 300、530 和 630°C 下退火 5 小时。使用SEM（扫描电子显微镜）、TF-XRD（薄膜X射线衍射）和接触角测角法表征所得纳米管层。成纤维细胞活力通过 CellTiter-Blue 方法确定，细胞毒性通过乳酸脱氢酶测试确定，并通过扫描电子显微镜分析细胞形态。此外，通过荧光显微镜分析细胞粘附、增殖和形态。结果表明，纳米管结晶度的改变可以提供有利的表面成纤维细胞生长，特别是在 530 和 630 °C 退火的基材上，表明这些特性为生物医学植入物提供了有利的模板。