Wheat is a foremost food grain of Pakistan and occupies a vital position in agricultural policies of the country. Wheat demand will be increased by 60% by 2050 which is a serious concern to meet this demand. Conventional breeding approaches are not enough to meet the demand of growing human population. It is paramount to integrate underutilized genetic diversity into wheat gene pool through efficient and accurate breeding tools and technology. In this study, we present the genetic analysis of a 312 diverse pre-breeding lines using DArT-seq SNPs seeking to elucidate the genetic components of emergence percentage, heading time, plant height, lodging, thousand kernel weight, and yield (Yd) which resulted in detection of 201 significant (p value < 10⁻³) and 61 highly significant associations (p value < 1.45 × 10⁻⁴). More importantly, chromosomes 1B and 2A carried loci linked to Yd in two different seasons, and an increase of up to 8.20% is possible in Yd by positive allele mining. We identified seven lines with > 4 positive alleles for Yd whose pedigree carried Aegilops squarrosa as one of the parents providing evidence that Aegilops species, apart from imparting resistance against biotic stresses, may also provide alleles for yield enhancement.

小麦是巴基斯坦的首要粮食，在该国农业政策中占有重要地位。到 2050 年，小麦需求将增加 60%，这是满足这一需求的一个严重问题。传统的育种方法不足以满足不断增长的人口需求。通过高效准确的育种工具和技术将未充分利用的遗传多样性整合到小麦基因库中至关重要。在这项研究中，我们使用 DArT-seq SNP 对 312 个不同的预育系进行了遗传分析，旨在阐明出苗率、抽穗时间、株高、倒伏、千粒重和产量 (Yd) 的遗传成分。导致检测到 201 个显着（p值 < 10 ^-3) 和 61 个高度显着的关联 ( p值 < 1.45 × 10 ^-4 )。更重要的是，染色体1B和2A在两个不同的季节携带与Yd连锁的基因座，通过正等位基因挖掘，Yd可能增加高达8.20%。我们确定了 7 个 Yd 具有 > 4 个阳性等位基因的品系，其谱系携带Aegilops squarerosa作为亲本之一，提供证据表明Aegilops物种除了赋予生物胁迫抗性外，还可以提供用于提高产量的等位基因。