Enzymes play an imminent role as biocatalyst for various biotechnological applications as well as production of biodiesel. Here, we focused on the preparation of biosupport materials (polyvinyl alcohol/AlgNa) and their use in immobilization of lipase from Pseudomonas cepacia. Lipase was successfully immobilized onto polyvinyl alcohol/AlgNa in the form of biosupport materials by entrapment method. The mechanical strength, swelling ratio, thermal properties, optimum temperature and pH, lipase loading, leaching, immobilization yields and activity, characterization of the support materials were performed. The optimized pH and temperature for free lipase were 8.0 and 40°C, respectively, while the best pH and temperature for polyvinyl alcohol/AlgNa immobilized lipase were 8.0 and 50°C. 73.12% of the initial activity was retained for the immobilized biosupport catalyst in 6 cycles. The biosupport catalyst beads showed a fascinating degree of immobilized lipase activity along with high immobilization yield. The highest immobilized lipase activity and loading efficiency found to be 87.28 (U/g) and 55.2%, respectively. SEM analysis confirms the development of macro-porous structure from the combination of alginate. No sharp chemical interaction was observed in the behaviour of the functional groups of polyvinyl alcohol and AlgNa in the polyvinyl alcohol/sodium alginate blends, which were confirmed from Fourier transform infrared (FTIR) spectra. The immobilized biosupport catalysts are easily separable, recyclable and could be frequently used for transesterification.

中文翻译：

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优化的生物支持材料（聚乙烯醇/ AlgNa）固定化脂肪酶的性能改善及其表征

酶在各种生物技术应用以及生物柴油生产中作为生物催化剂的作用迫在眉睫。在这里，我们专注于生物支撑材料（聚乙烯醇/ AlgNa）的制备及其在固定洋葱假单胞菌脂肪酶中的应用。通过包埋法将脂肪酶成功地以生物支持材料的形式固定在聚乙烯醇/ AlgNa上。进行了机械强度，溶胀率，热性能，最适温度和pH，脂肪酶负载，浸出，固定化产率和活性，载体材料的表征。游离脂肪酶的最佳pH和温度分别为8.0和40°C，而聚乙烯醇/ AlgNa固定化脂肪酶的最佳pH和温度为8.0和50°C。固定的生物载体催化剂在6个循环中保留了73.12％的初始活性。生物载体催化剂珠粒显示出令人着迷的固定化脂肪酶活性以及高固定化产率。最高的固定化脂肪酶活性和负载效率分别为87.28（U / g）和55.2％。SEM分析证实了藻酸盐的结合形成了大孔结构。聚乙烯醇/海藻酸钠共混物中聚乙烯醇和AlgNa的官能团的行为未观察到剧烈的化学相互作用，这已通过傅里叶变换红外（FTIR）光谱得到了证实。固定化的生物载体催化剂易于分离，可回收，并可经常用于酯交换反应。