Podophyllum hexandrum is a popular medicinal plant endemic to Himalayas. In the present investigation, we report an efficient mass propagation protocol and Podophyllotoxin (PTOX) production in in vitro-derived plants of P. hexandrum, cultured on MS medium containing various plant growth regulators and polyamines. A combination of BA (1.5 mg/L), IAA (0.2 mg/L) and spermidine (20 mg/L) produced the highest number of multiple shoots per explant (23 shoots) after 6 weeks of culture; the regenerated shoots were elongated on the same medium. The elongated-shoots were rooted on root induction medium (half strength MS medium) supplemented with IBA (1.5 mg/L) and putrescine (15 mg/L). The rooted plantlets were successfully hardened and acclimatized with a survival rate of 68% in the greenhouse. The highest content of PTOX (4.23 mg/g DW) was recorded in in vitro derived roots followed by leaves when compared to field-grown parent plants. The present system offers the possibility to use in vitro culture techniques for mass propagation and PTOX production for commercial utilization.

中文翻译：

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多胺在六叶鬼臼体外再生和鬼臼毒素产生中的作用

鬼臼（Podophyllum hexandrum）是喜马拉雅山特有的流行药用植物。在本研究中，我们报告了在六味子藻的体外衍生植物中有效的大量繁殖方案和鬼臼毒素（PTOX）的产生，在含有各种植物生长调节剂和多胺的MS培养基上培养。培养6周后，BA（1.5 mg / L），IAA（0.2 mg / L）和亚精胺（20 mg / L）的组合产生了最高数量的外植体多发芽（23枝）；再生的芽在相同的培养基上伸长。伸长的枝条扎根于补充有IBA（1.5 mg / L）和腐胺（15 mg / L）的根诱导培养基（半强度MS培养基）。生根的小苗已成功变硬并适应了温室中的成活率，达到68％。与田间生长的亲本植物相比，PTOX的最高含量（4.23 mg / g DW）记录在体外衍生的根系中，其次是叶片。本系统提供了体外使用的可能性 大规模繁殖和PTOX生产的商业化养殖技术。