In the present study, amino-functionalised mesoporous silica microspheres were utilised as support for the covalent immobilisation of Candida antarctica lipase B (CaLB) for the subsequent production of 2,5-furandicarboxylic acid (FDCA) from 2,5-diformylfuran (DFF). Under the optimised operating conditions of pH 6.5, particle/enzyme ratio of 1.25:1.0 and glutaraldehyde concentration of 4 mM, a maximum CaLB immobilisation yield of 82.4% on silica microspheres was obtained in 12.25 h. The immobilised CaLB was used for the synthesis of alkyl esters, which were utilised along with hydrogen peroxide for FDCA synthesis. The biocatalytic conversion of 30 mM DFF dictated a 77–79% FDCA in 48 h at 30°C; where the turnover number and turnover frequency of immobilised CaLB were 6220.73 mol mol ⁻¹ and 129.59 h ⁻¹ , respectively, for ethyl acetate, against 6297.65 mol mol ⁻¹ and 131.2 h ⁻¹ , respectively, for ethyl butyrate. Upon examining the operational stability, the immobilised CaLB exhibited high stability till five cycles of FDCA production.

中文翻译：

---

用于固定南极念珠菌脂肪酶 B 的氨基官能化介孔二氧化硅微球——在 2,5-呋喃二甲酸的绿色生产中的应用

在本研究中，氨基官能化的介孔二氧化硅微球被用作共价固定南极念珠菌脂肪酶 B (CaLB) 用于随后从 2,5-二甲酰基呋喃 (DFF) 生产 2,5-呋喃二甲酸 (FDCA)。在 pH 6.5、颗粒/酶比为 1.25:1.0 和戊二醛浓度为 4 mM 的优化操作条件下，在 12.25 小时内，二氧化硅微球上的最大 CaLB 固定产率为 82.4%。固定化 CaLB 用于合成烷基酯，与过氧化氢一起用于 FDCA 合成。30 mM DFF 的生物催化转化要求 30°C 下 48 小时内 FDCA 为 77–79%；其中，固定化 CaLB 的转换次数和转换频率分别为 6220.73 mol mol ^-1和 129.59 h ^-1 ，乙酸乙酯为 6297.65 mol mol ^-1和 131.2 h ^-1 ，分别为丁酸乙酯。在检查操作稳定性后，固定化的 CaLB 在 FDCA 生产的五个周期内表现出高稳定性。