Background: The determination of mancozeb, a fungicide extensively used in agriculture, is a challenge, due to the nature of the compound, a manganese and zinc complex of ethylenebis dithiocarbamate and because of the general instability of the dithiocarbamates.

Methods: Mancozeb was analyzed in a GC-EI-MS system after derivatization by CE-UV with detection at 280 nm and in LC-ESI-MS-MS in MRM mode.

Results: A comparative study of the performance of three different techniques for the detection of mancozeb was explored, highlighting the advantages and drawbacks of them. The limits of detection and quantification of the techniques were determined; the repeatability was assessed, showing values of relative standard deviation. Gas chromatography, although very sensitive, was not reproducible enough due to fast degradation of the derivatization product, whereas capillary electrophoresis-UV showed problems in run-to-run reproducibility which had the worst limit of detection. LC coupled with tandem mass spectrometry was the most reliable and precise technique and was able to determine the main degradation product of Mancozeb, at the same time. The proposed LC procedure was verified by applying it to a commercial formulation, a fungicide of known concentration, and to Italian white grapes treated with the formulation sprayed during cultivation.

Conclusion: Thanks to the simplified sample handling, the proposed method resulted to be simple, fast, green, economic, and suitable for residue analysis in grapes and other fruits. Finally, the method was compared with other similar investigations.

背景：由于化合物的性质，亚乙基双二硫代氨基甲酸酯的锰和锌配合物以及二硫代氨基甲酸酯的一般不稳定性，测定广泛用于农业的杀真菌剂mancozeb是一项挑战。

方法：通过CE-UV衍生化并在MRM模式下在LC-ESI-MS-MS中进行280-nm检测，然后在GC-EI-MS系统中对Mancozeb进行分析。

结果：对三种不同技术检测锰锌的性能进行了比较研究，突出了它们的优缺点。确定了技术检测和定量的极限；评估重复性，显示相对标准偏差的值。气相色谱法虽然非常灵敏，但由于衍生化产物的快速降解而无法充分再现，而毛细管电泳-UV则显示了每次运行的重现性方面的问题，其检测限最差。LC与串联质谱联用是最可靠，最精确的技术，能够同时测定Mancozeb的主要降解产物。建议的LC程序已通过将其应用于商业配方而得到验证，

结论：由于简化了样品处理，所提出的方法简单，快速，绿色，经济，并且适用于葡萄和其他水果中的残留物分析。最后，将该方法与其他类似研究进行了比较。