Acute liver injury is a serious threat to human health. Complementary therapy including a traditional Chinese herb has been used for the prevention and treatment of liver injuries. Schisandrae sphenantherae fructus (Schisandra) is the mature dry fruit of Schisandra sphenanthera Rehd. et Wils. Wuzhi capsule, a preparation containing Schisandra and its main component anwulignan, is used to treat hepatitis and hepatic insufficiency caused by viruses and drugs in the clinic. However, to date, there has been little study to reveal the effect of anwulignan in the protection of the liver. Therefore, in this study, we hypothesized that anwulignan could protect carbon tetrachloride (CCl₄)-induced acute liver injury in mice. Anwulignan was shown to reduce significantly the liver index, decrease liver histopathological injury, decrease the serum level of aspartate aminotransferase and alanine aminotransferase, increase the activities of superoxide dismutase (SOD) and glutathione peroxidase, reduce liver malondialdehyde content, and downregulate the expression levels of interleukin (IL)-6, IL-1β, and tumor necrosis factor-α in the liver tissue, as well as the protein expression levels of receptor-interacting serine/threonine-protein kinase 1 (RIPK1), RIPK3, and phosphorylated mixed lineage kinase domain-like protein. All these results suggest that anwulignan can alleviate the CCl₄-induced acute liver injury in mice, which may be related to its antioxidant, anti-inflammation, and inhibition of liver cell necroptosis effects.

急性肝损伤是对人类健康的严重威胁。包括传统中草药在内的辅助疗法已用于预防和治疗肝损伤。五味子sphenantherae枸杞（五味子）是成熟的干果华中五味子甜茶。et Wils。五味胶囊是一种含有五味子及其主要成分安乌利格南的制剂，用于治疗临床上由病毒和药物引起的肝炎和肝功能不全。然而，迄今为止，很少有研究揭示安乌利格南在保护肝脏中的作用。因此，在本研究中，我们假设安乌利肝素可以保护四氯化碳（CCl ₄）诱发小鼠急性肝损伤。已显示Anwulignan可显着降低肝脏指数，减少肝脏组织病理学损伤，降低血清天冬氨酸转氨酶和丙氨酸转氨酶水平，增加超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶的活性，降低肝脏丙二醛含量并下调其表达水平肝组织中的白介素（IL）-6，IL-1β和肿瘤坏死因子-α，以及与受体相互作用的丝氨酸/苏氨酸蛋白激酶1（RIPK1），RIPK3和磷酸化混合谱系的蛋白表达水平激酶结构域样蛋白。所有这些结果表明安乌利格南可以减轻CCl ₄引起的小鼠急性肝损伤，可能与其抗氧化剂，抗炎作用和抑制肝细胞坏死作用有关。