The carbon catabolite repressor 4-negative on TATA (CCR4-NOT) complex, abbreviated CNOT, has deadenylation and 3′-5′ exonuclease activity, mediates deadenylation in the degradation of RNA, initiates the exonuclease degradation pathway, and participates in tumor gene regulation. CNOT proteins comprise a family of global transcriptional regulators that are evolutionarily conserved in eukaryotic cells. Several subunit types of the CNOT complex have been discovered; however, little is known about the role of different subunits in tumorigenesis and development. We observed overexpression of CNOT1–11 in liver cancer and correlations with clinicopathological characteristics. The expression of some CNOTs subunits was associated with histological grades, lymph node metastasis, and tumor stages in patients with hepatocellular carcinoma (HCC). Our data suggested that some CNOTs can be used as predictors of poor prognosis in HCC patients. At the same time, we conducted an analysis of CNOTs mutations in HCC patients. Moreover, we selected CNOT6, CNOT10, and CNOT11 for protein interaction network analysis and Gene Ontology enrichment analysis to investigate their related biological processes and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Finally, the results of western blot and quantitative reverse transcription-PCR (qRT-PCR) experiments were consistent with the database findings. Results of this study suggest that CNOT6, CNOT10, and CNOT11, acting as regulators of transcription, may play an important role in the development of HCC and may serve as biological markers in the diagnosis and prognosis of HCC.

中文翻译：

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肝细胞癌中CNOT家族基因表达，临床病理特征及预后价值分析

TATA（CCR4-NOT）复合物上的碳分解代谢阻遏物4阴性，缩写为CNOT，具有去烯基化和3'-5'核酸外切酶活性，在RNA降解中介导去烯基化，启动核酸外切酶降解途径，并参与肿瘤基因调控。CNOT蛋白包含一个在真核细胞中进化保守的全局转录调节因子家族。已经发现了CNOT复合体的几种亚基类型。然而，关于不同亚基在肿瘤发生和发展中的作用知之甚少。我们观察到肝癌中CNOT1-11的过表达及其与临床病理特征的关系。一些CNOT的表达亚型与肝细胞癌（HCC）患者的组织学分级，淋巴结转移和肿瘤分期有关。我们的数据表明，某些CNOT可作为HCC患者预后不良的预测指标。同时，我们对HCC患者的CNOTs突变进行了分析。此外，我们选择了CNOT6，CNOT10和CNOT11进行蛋白质相互作用网络分析和基因本体论富集分析，以研究它们的相关生物学过程以及《京都议定书》的基因和基因组百科全书（KEGG）途径。最后，蛋白质印迹和定量逆转录PCR（qRT-PCR）实验的结果与数据库的发现是一致的。这项研究的结果表明，CNOT6，CNOT10和CNOT11充当转录调节因子，