Mesenchymal stem cells (MSCs) may improve the treatment of acute respiratory distress syndrome (ARDS). However, few studies have investigated the effects of mechanically stretched -MSCs (MS-MSCs) in in vitro models of ARDS. The aim of this study was to evaluate the potential therapeutic effects of MS-MSCs on pulmonary microvascular endothelium barrier injuries induced by LPS. We introduced a cocultured model of pulmonary microvascular endothelial cell (EC) and MSC medium obtained from MSCs with or without mechanical stretch. We found that Wright-Giemsa staining revealed that MSC morphology changed significantly and cell plasma shrank separately after mechanical stretch. Cell proliferation of the MS-MSC groups was much lower than the untreated MSC group; expression of cell surface markers did not change significantly. Compared to the medium from untreated MSCs, inflammatory factors elevated statistically in the medium from MS-MSCs. Moreover, the paracellular permeability of endothelial cells treated with LPS was restored with a medium from MS-MSCs, while LPS-induced EC apoptosis decreased. In addition, protective effects on the remodeling of intercellular junctions were observed when compared to LPS-treated endothelial cells. These data demonstrated that the MS-MSC groups had potential therapeutic effects on the LPS-treated ECs; these results might be useful in the treatment of ARDS.

中文翻译：

---

机械拉伸间充质干细胞可以减少LPS诱导的损伤对肺微血管内皮屏障的影响。

间充质干细胞（MSC）可以改善急性呼吸窘迫综合征（ARDS）的治疗。但是，很少有研究研究体外机械拉伸的-MSC（MS-MSC）的作用ARDS模型。这项研究的目的是评估MS-MSC对LPS诱导的肺微血管内皮屏障损伤的潜在治疗作用。我们介绍了肺微血管内皮细胞（EC）和MSC培养基（从具有或不具有机械拉伸的MSC）获得的共培养模型。我们发现，赖特-吉姆萨染色显示，机械拉伸后，MSC形态发生了显着变化，细胞血浆分别收缩。MS-MSC组的细胞增殖远低于未处理的MSC组。细胞表面标志物的表达没有明显改变。与未经处理的MSCs的培养基相比，MS-MSCs的培养基中的炎症因子统计学上升高。此外，LPS处理的内皮细胞的细胞旁通透性已通过MS-MSCs的培养基恢复，LPS诱导的EC凋亡减少。另外，与LPS处理的内皮细胞相比，观察到对细胞间连接重塑的保护作用。这些数据表明，MS-MSC组对经LPS处理的EC具有潜在的治疗作用。这些结果可能对ARDS的治疗有用。