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Real-Time Multi-Angle Projection Imaging of Biological Dynamics
bioRxiv - Biophysics Pub Date : 2020-10-29 , DOI: 10.1101/2020.10.29.355917
Bo-Jui Chang , Etai Sapoznik , Theresa Pohlkamp , Tamara S. Terrones , Erik S. Welf , James D. Manton , Philippe Roudot , Kayley Hake , Lachlan Whitehead , Andrew G. York , Kevin M. Dean , Reto Fiolka

We introduce a cost-effective and easily implemented scan unit which enables any camera-based microscope to perform projection imaging from diverse viewing angles. We demonstrate this capability on Lattice Light-Sheet and Oblique Plane Microscopy by rapidly delivering projection images with an uncompromised lateral resolution and high optical contrast. By imaging the sample from one or multiple perspectives, our method enables visualization of rapid biological processes, real time stereoscopic imaging as well as three-dimensional particle localization throughout a cellular volume from just two images. Furthermore, because our projection imaging technique provides intuitive three-dimensional renderings in real-time, it improves microscope usability, allows users to more-readily optimize instrument performance and identify biological phenomena of interest on-the-fly, while also reducing data overhead by a factor of >100. We leverage our rapid projection method to image cancer cell morpho-dynamics and calcium signaling in cultured neurons, to perform three-dimensional localization of genetically encoded nanoparticles, as well as to image orthogonal views of an embryonic Zebrafish heart simultaneously.

中文翻译:

生物动力学实时多角度投影成像

我们推出了一种经济高效且易于实施的扫描单元,该扫描单元使任何基于相机的显微镜都能从不同的视角执行投影成像。我们通过快速交付投影图像而毫不妥协的横向分辨率和高光学对比度,证明了在格子光片和斜面显微镜上的这种能力。通过从一个或多个角度对样品进行成像,我们的方法可以从两个图像中快速观察生物过程,实时立体成像以及整个细胞体积中的三维粒子定位。此外,由于我们的投影成像技术可以实时提供直观的三维渲染,因此可以提高显微镜的可用性,允许用户更轻松地优化仪器性能并即时识别感兴趣的生物现象,同时还将数据开销减少100倍以上。我们利用我们的快速投影方法对培养的神经元中的癌细胞形态动力学和钙信号进行成像,对基因编码的纳米粒子进行三维定位,同时对胚胎斑马鱼心脏的正交视图进行成像。
更新日期:2020-10-30
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