Cytochrome P450 monooxygenases (P450s) play significant roles in protecting organisms from abiotic stress damage. Here, we report the sequence and characterization of a P450s gene (AccCYP4AV1), isolated from Apis cerana cerana Fabricius. The open reading frame of AccCYP4AV1 is 1506 base pairs long and encodes a predicted protein of 501 amino acids and 57.84 kDa, with an isoelectric point of 8.67. Real-time quantitative polymerase chain reaction (RT-qPCR) analysis indicated that AccCYP4AV1 is more highly expressed in the midgut than in other tissues. In addition, the highest expression occurs in newly emerged adult workers, followed by the first instar of the larval stage. In addition, the expression of the AccCYP4AV1 was upregulated by low temperature (4 °C), ultraviolet radiation, hydrogen peroxide, paraquat, and dichlorvos treatments. In contrast, AccCYP4AV1 transcription was downregulated by other abiotic stress conditions: exposure to increased temperature (44 °C), deltamethrin, cadmium chloride, and mercury (II) chloride. Moreover, when AccCYP4AV1 was knocked-down by RNA interference, the results suggested that multiple antioxidant genes (AccsHSP22.6, AccSOD2, AccTpx1, and AccTpx4) were downregulated and antioxidant genes AccGSTO1 and AccTrx1 were upregulated. The activity levels of peroxidase and catalase were upregulated in the AccCYP4AV1-knocked-down samples, compared with those in the control groups. These findings suggest that the AccCYP4AV1 protein might be involved in the defense against abiotic stress damage.

中文翻译：

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一种新的 P450s 基因 (AccCYP4AV1) 的鉴定及其在 Apis cerana cerana Fabricius 抗非生物胁迫中的作用

细胞色素 P450 单加氧酶 (P450s) 在保护生物体免受非生物胁迫损伤方面发挥着重要作用。在这里，我们报告了一个 P450s 基因的序列和特征（ACCCYP4AV1)，从中华蜜蜂法布里修斯。的开放阅读框架ACCCYP4AV1长度为 1506 个碱基对，编码 501 个氨基酸和 57.84 kDa 的预测蛋白质，等电点为 8.67。实时定量聚合酶链反应 (RT-qPCR) 分析表明，ACCCYP4AV1在中肠中比在其他组织中表达更高。此外，新出现的成年工人中表达最高，其次是幼虫阶段的第一龄。此外，表达式ACCCYP4AV1通过低温（4°C）、紫外线辐射、过氧化氢、百草枯和敌敌畏处理上调。相比之下，ACCCYP4AV1转录被其他非生物胁迫条件下调：暴露于升高的温度（44°C）、溴氰菊酯、氯化镉和氯化汞（II）。此外，当ACCCYP4AV1被RNA干扰敲低，结果表明多个抗氧化基因（ACCHSP22.6,AccSOD2,AccTpx1， 和AccTpx4) 被下调和抗氧化基因ACCGSTO1和帐户Trx1被上调。过氧化物酶和过氧化氢酶的活性水平在ACCCYP4AV1- 与对照组相比，击倒样本。这些发现表明，累积CYP4AV1 蛋白可能参与防御非生物胁迫损伤。