ABSTRACT

In this research, the Polycaprolactone/Gelatin/Hydroxyapatite nanoparticles (PGH) scaffold was successfully fabricated through electrospinning technique and the physical, chemical, and biological properties were assessed. The morphological observation exhibited relatively bead-free uniform fibers and the fiber diameter, pore size, and porosity were 616 nm, 9 μm, and 86 %, respectively.Next, the stem cells isolated from human exfoliated deciduous teeth were cultured on the scaffolds and their proliferation and differentiation to osteoblast were examined through Alizarin red staining, MTT assay, q-RT-PCR technique, and ALP activity. Moreover, the efficacy of stem cell-seeded scaffolds to enhance the regeneration of Wistar rats’ cranial bone defects was assessed. The results revealed that the PGH scaffold was completely biocompatible and it improved the stem cells' adhesion, proliferation, and differentiation significantly. Through thein vivo studies, it was revealed that the stem cell-seeded scaffolds could significantly accelerate the repair of the cranial bone defect.

摘要

本研究通过静电纺丝技术成功制备了聚己内酯/明胶/羟基磷灰石纳米粒子（PGH）支架，并对其物理、化学和生物学特性进行了评估。形态学观察显示出相对无珠的均匀纤维，纤维直径、孔径和孔隙率分别为616 nm、9 μm和86%。接下来，从人脱落的乳牙中分离出的干细胞在支架上培养，通过茜素红染色、MTT法、q-RT-PCR技术和ALP活性检测它们的增殖和向成骨细胞的分化。此外，还评估了干细胞接种支架增强 Wistar 大鼠颅骨缺损再生的功效。结果表明，PGH支架具有完全的生物相容性，显着改善了干细胞的粘附、增殖和分化。通过体内 研究表明，干细胞种子支架可以显着加速颅骨缺损的修复。