ABSTRACT

To assess the m⁶A methylome in the anterior capsule of the lens of high myopia patients. MeRIP-seq and RNA-seq were performed to identify differences in the m⁶A methylomes and gene expression between anterior capsule of the lens of simple nuclear cataract patients (N) and nuclear cataract patients with high myopia (G). Expression of m⁶A-related enzymes was confirmed by quantitative real-time-PCR. ALKBH5 was downregulated in G. The observed m⁶A peak was identical to the conserved RRACH gmotif and was markedly correlated with two distinct coordinates. Differentially methylated genes were enriched in some pathways regulating the formation of extracellular matrix. These findings suggest that upregulation of m⁶A methylation may change fundus anatomy by regulating the composition of the extracellular matrix through encoding protein.

摘要

评估高度近视患者晶状体前囊中的m ^{6 A 甲基化组。}进行 MeRIP-seq 和 RNA-seq 以确定单纯性核性白内障患者 (N) 和高度近视的核性白内障患者 (G) 晶状体前囊之间m ^{6 A 甲基化组和基因表达的差异。}m ⁶ A 相关酶的表达通过定量实时PCR确认。ALKBH5 在 G 中被下调。观察到的 m ⁶ A 峰与保守的 RRACH gmotif 相同，并且与两个不同的坐标显着相关。差异甲基化基因在一些调节细胞外基质形成的途径中富集。这些发现表明 m ^6的上调甲基化可以通过编码蛋白质来调节细胞外基质的组成，从而改变眼底解剖结构。