ABSTRACT

Our objective was to study the differentially expressed genes (DEGs) of apple (Malus. sp.) rootstock with different gravitropic setpoint angles (GSAs), during the formation of stem-root GSAs. The annual shoots of eight years hybrid seedlings of Malus pumila‘Ralls’ and M. sp. were used as experimental materials, and softwood cutting was carried out in mid-May. Different strains of adventitious root GSA were screened and shoot cuttings were carried out again the following year. The cuttings were randomly selected at 24 day after cutting, and then the transcriptome analysis was carried out. The results show that: (1) according to the difference of stem-root GSA of cutting seedlings, the seedlings of apple rootstock were divided into 3 groups: deep root type (SC), intermediate root type (ZC) and shallow root type (QC). (2) Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that 2613 genes from shallow- and deep-rooted rootstock, 2091 from deep- and intermediate-rooted rootstock, and 3114 from intermediate- and shallow-rooted rootstock, were annotated into 125 pathways. Among these pathways, plant hormone signal transduction, biosynthesis of secondary metabolites, and phenylpropanoid biosynthesis pathways were significantly enriched (P < 0.01).

摘要

我们的目标是研究苹果（的差异表达的基因（DEGS）苹果茎根GSA的形成过程中。物种）砧木具有不同设定值向地角（GSA的）。海棠（Malus pumila） “ Ralls”和 M的八年杂种幼苗的年梢 。sp。用作实验材料，5月中旬进行了软木切割。筛选了不定根GSA的不同菌株，并于次年再次进行了cutting插。插条后第24天随机选择插条，然后进行转录组分析。结果表明：（1）根据插秧苗的茎根GSA的差异，将苹果砧木幼苗分为三类：深根型（SC），中根型（ZC）和浅根型（质量控制）。（2）《京都基因与基因组百科全书》（KEGG）分析显示，将浅根和深根砧木的2613个基因，深根和中根砧木的2091个基因以及中根和浅根砧木的3114个基因注释为125条途径。在这些途径中，