Abstract

Hypertension is a disease, which in spite of existing treatments continues to have high morbidity and mortality, which suggests that there are other mechanisms involved in this pathology. In this sense, the orphan receptors are G protein-coupled receptor associated with various pathologies such as GPR99 which has been linked to mice develop left ventricular hypertrophy induced by blood pressure overload while GPR107 with patients with idiopathic pulmonary arterial hypertension. For this reason, the aim of this work was to study if the expression of the orphan receptors GPR99 and GPR107 are modified by arterial hypertension. Male SHR and WKY rats of 6–8 and 10–12 weeks old were used. The weight, systolic blood pressure and heart rate were measured, as well as the mRNA of the receptors GPR99 and GPR107 in the aorta, kidney, heart and brain by RT-PCR, also was realized an in silico analysis to predict which G protein could be coupled the orphan receptor GPR107. Our results showed that receptors GPR99 and GPR107 are expressed in the analyzed tissues and their expression profile tends to change at different ages and with the development of hypertension, for the other hand, the bioinformatics analysis for GPR107 showed that is coupled to Gi protein. Therefore, we do not rule out that GPR99 and GPR107 could be involved in the pathophysiology of hypertension and could be used as targets therapeutic in hypertension.

摘要

高血压是一种疾病，尽管有现有的治疗方法，但其发病率和死亡率仍然很高，这表明该病理学中还涉及其他机制。从这个意义上说，孤儿受体是与各种病理相关的 G 蛋白偶联受体，例如 GPR99 与小鼠因血压超负荷而导致左心室肥大有关，而 GPR107 与特发性肺动脉高压患者有关。出于这个原因，这项工作的目的是研究孤儿受体 GPR99 和 GPR107 的表达是否被动脉高血压所改变。使用 6-8 和 10-12 周龄的雄性 SHR 和 WKY 大鼠。通过 RT-PCR 测量体重、收缩压和心率，以及主动脉、肾脏、心脏和大脑中受体 GPR99 和 GPR107 的 mRNA，还实现了计算机分析，以预测哪种 G 蛋白可以与孤儿受体 GPR107 偶联。我们的研究结果表明，受体 GPR99 和 GPR107 在分析的组织中表达，并且它们的表达谱在不同年龄和随着高血压的发展而发生变化，另一方面，GPR107 的生物信息学分析表明它与 Gi 蛋白偶联。因此，我们不排除 GPR99 和 GPR107 可能参与高血压的病理生理过程，并可作为治疗高血压的靶点。我们的结果表明受体 GPR99 和 GPR107 在分析的组织中表达，并且它们的表达谱在不同年龄和随着高血压的发展而趋于变化，另一方面，GPR107 的生物信息学分析表明它与 Gi 蛋白偶联。因此，我们不排除 GPR99 和 GPR107 可能参与高血压的病理生理过程，并可作为治疗高血压的靶点。我们的结果表明受体 GPR99 和 GPR107 在分析的组织中表达，并且它们的表达谱在不同年龄和随着高血压的发展而趋于变化，另一方面，GPR107 的生物信息学分析表明它与 Gi 蛋白偶联。因此，我们不排除 GPR99 和 GPR107 可能参与高血压的病理生理过程，并可作为治疗高血压的靶点。