Abstract

Purpose

This study mainly aimed to explore the influences of Calcium Voltage-Gated Channel Subunit Alpha1 B (CACNA1B) on the development of breast cancer and the related mechanism.

Materials and methods

The information of patients with breast cancer from TCGA database was used for analyses of CACNA1B expression and its prognostic value. Loss- and gain- of functions of CACNA1B were conducted in MCF7 and Bcap-37 cells, respectively. CCK-8, colony formation and transwell assays were applied for evaluating the cell viability and motility. Western blot was used for protein expression detection.

Results

We revealed that highly expressed CACNA1B in breast cancer tissues was related to poor prognosis according to the data gained from TCGA database. The outcomes of functional assays showed that depletion of CACNA1B restrained MCF7 cell growth, invasion and migration and high-expression of CACNA1B fortified the growth, invasion and migration in Bcap-37 cells. Finally, we manifested that silencing CACNA1B obviously raised the protein expression level of E-cadherin and reduced the protein levels of Cyclin D1, N-cadherin and Snail in MCF7 cells, whilst, over-expression of CACNA1B reduced the level of E-cadherin and increased the expression of Cyclin D1, N-cadherin and Snail in Bcap-37 cells.

Conclusions

These results identified CACNA1B as a forwarder of the growth, invasion and migration in breast cancer cells.

中文翻译：

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CACNA1B 通过调节细胞周期蛋白 D1 和 EMT 促进乳腺癌细胞的生长和迁移：CACNA1B 在乳腺癌中的意义

摘要

目的

本研究主要探讨钙电压门控通道亚基α1B（CACNA1B）对乳腺癌发生发展的影响及相关机制。

材料和方法

TCGA数据库中的乳腺癌患者信息用于分析CACNA1B的表达及其预后价值。CACNA1B 功能的丧失和获得分别在 MCF7 和 Bcap-37 细胞中进行。CCK-8、集落形成和transwell测定用于评估细胞活力和运动性。Western印迹用于蛋白质表达检测。

结果

根据从 TCGA 数据库获得的数据，我们发现乳腺癌组织中高表达的 CACNA1B 与预后不良有关。功能测定结果表明，CACNA1B 的消耗抑制了 MCF7 细胞的生长、侵袭和迁移，而 CACNA1B 的高表达增强了 Bcap-37 细胞的生长、侵袭和迁移。最后，我们发现沉默 CACNA1B 明显提高了 MCF7 细胞中 E-cadherin 的蛋白表达水平，降低了 Cyclin D1、N-cadherin 和 Snail 的蛋白水平，而 CACNA1B 的过表达降低了 E-cadherin 和增加 Bcap-37 细胞中 Cyclin D1、N-cadherin 和 Snail 的表达。

结论

这些结果将 CACNA1B 鉴定为乳腺癌细胞生长、侵袭和迁移的转发器。