Abstract

This study was focused to determine an individual and combined effect of mycotoxin citrinin (CIT) and two compounds of the stilbene family- resveratrol (RES) and his dimethyl ether analogue pterostilbene (PTE) which have many health benefits. As a model the human adenocarcinoma cell line HT-29 was used which may exhibits the properties of small intestine cells. Viability, plasma membrane integrity, lysosomal functionality, intracellular production of superoxide anions and superoxide dismutase activity were examined. The results indicate that concentrations of 50 and 100 μg/mL of the tested compounds were cytotoxic in mostly monitored parameters and probably caused apoptosis. HT-29 cells were more sensitive to PTE than to RES with a higher antioxidant effect of PTE than RES, which may be caused by its chemical structure. Both stilbenes at medium doses act as effective superoxide anions scavengers leading to reduction of oxidative stress and consequent cell damage. The nontoxic concentration of RES (25 µg/mL) protects the HT-29 cell line faced to the toxicity of CIT at 25 µg/mL by increasing viability of cells and by reducing the superoxide production induced by CIT concentrations of 12.5 µg/mL and 25 µg/mL.

摘要

这项研究的重点是确定霉菌毒素西林蛋白（CIT）和二苯乙烯家族白藜芦醇（RES）和他的二甲醚类似物翼戊二烯（PTE）的两种化合物的个体和综合作用，它们具有许多健康益处。作为模型，使用人腺癌细胞系HT-29，该细胞系可能表现出小肠细胞的特性。检查了活力，质膜完整性，溶酶体功能，细胞内超氧化物阴离子的产生和超氧化物歧化酶的活性。结果表明，浓度为50和100μg/ mL的被测化合物在大多数监测参数中具有细胞毒性，并可能引起细胞凋亡。HT-29细胞对PTE比对RES更敏感，PTE的抗氧化作用比RES高，这可能是由于其化学结构引起的。两种中剂量的斯蒂芬苯酯均作为有效的超氧化物阴离子清除剂，导致氧化应激的降低和对细胞的损害。RES的无毒浓度（25 µg / mL）通过增加细胞活力并减少12.5 µg / mL CIT浓度引起的超氧化物生成，从而保护HT-29细胞面对25 µg / mL CIT毒性。 25 µg / mL。