International Journal of Neuroscience ( IF 2.2 ) Pub Date : 2021-03-04 , DOI: 10.1080/00207454.2020.1829618 Jiwu Liao 1 , Sisi Wang 1 , Haihua Yang 1 , Ping Ma 1 , Congrui Li 1 , Qiaoting Huang 1 , Jiyang Pan 1
Abstract
Objective
We aimed to investigate the expression levels of GABA and GABAA receptor α1 and α2 subunits in patients with major depressive disorder (MDD) during onset and remission.
Materials and Methods
48 patients with MDD during onset and 45 patients with MDD during remission who were treated in our university were selected. Moreover, the control group included 46 healthy volunteers recruited in the community. The depression and anxiety symptoms were assessed by Hamilton Depression (HAMD) Scale and Hamilton Anxiety (HAMA) Scale. ELISA was used to determine the serum GABA levels. The mRNA expression of GABAA receptor α1 and α2 subunits in peripheral blood were detected by RT-PCR.
Results
The expression levels of serum GABA and of GABAA receptor α1 and α2 subunits in MDD depression attack group were notably decreased in comparison with those in MDD remission group and control group ((4.10 ± 0.73) v.s. (5.91 ± 1.25) and (5.83 ± 1.17) umol/L, F = 5.61, p < 0.001; (0.53 ± 0.32) v.s. (0.91 ± 0.18) and (0.93 ± 0.21), F = 8.37, p < 0.001; (1.45 ± 0.86) v.s. (2.33 ± 1.49) and (2.28 ± 1.50), F = 8.23, p < 0.001). However, there were no marked difference in the levels of these three indices between the MDD remission group and the control group (p > 0.05). Serum GABA levels were negatively correlated with HAMA total score (r = −0.34, p = 0.02), HAMD total score (r = −0.46, p = 0.01) and depression core symptom score (r = −0.32, p = 0.03).
Conclusions
During the onset of MDD, there may be GABA neuronal dysfunction and abnormal expression of GABAA receptor subunits, and those changes showed a state change, which gradually returned to normal during remission.
中文翻译:
重度抑郁症患者发病和缓解期间GABAA受体α1和α2亚基的mRNA表达水平
摘要
客观的
我们旨在研究重度抑郁症 (MDD) 患者发病和缓解期间GABA 和 GABA A受体 α1 和 α2 亚基的表达水平。
材料和方法
选择在我校就诊的发病期MDD患者48例和缓解期MDD患者45例。此外,对照组包括在社区招募的46名健康志愿者。通过汉密尔顿抑郁(HAMD)量表和汉密尔顿焦虑(HAMA)量表评估抑郁和焦虑症状。ELISA用于测定血清GABA水平。RT-PCR检测外周血GABA A受体α1和α2亚基的mRNA表达。
结果
MDD抑郁发作组血清GABA和GABA A受体α1、α2亚基的表达水平较MDD缓解组和对照组明显降低((4.10±0.73)vs(5.91±1.25)和(5.83± 1.17) umol/L, F = 5.61, p < 0.001; (0.53 ± 0.32) vs (0.91 ± 0.18) and (0.93 ± 0.21), F = 8.37, p < 0.001; (1.45 ± 0.86) vs (2.33 ± 1.49) ) 和 (2.28 ± 1.50), F = 8.23, p < 0.001)。然而,MDD 缓解组和对照组在这三个指标的水平上没有显着差异(p > 0.05)。血清 GABA 水平与 HAMA 总分 (r = -0.34, p = 0.02)、HAMD 总分 (r = -0.46, p = 0.01) 和抑郁症核心症状评分 (r = -0.32, p = 0.03) 呈负相关。
结论
MDD发病期间可能出现GABA神经元功能障碍和GABA A受体亚基异常表达,这些变化表现为状态变化,缓解期逐渐恢复正常。