Extremely low-frequency electromagnetic field (ELF-EMF) exposure influences many biological systems; these effects are mainly related to the intensity, duration, frequency, and pattern of the ELF-EMF. In this study, exposure to square wave with 7.83±0.3 Hz (sweep step 0.1 Hz) was shown to inhibit the growth of B16F10 melanoma tumor cells. In addition, the distribution of the magnetic field was calculated by Biot-Savart Law and plotted using MATLAB. In vitro studies demonstrated a decrease in B16F10 cell proliferation and an increase of Ca2+ influx after 48 h of exposure to the square wave. Ca2+ influx was also partially blocked by inhibition of voltage-gated L- and T-type Ca2+ channels. The data confirmed that the specific time-varying ELF-EMF had an anti-proliferation effect on B16F10 cells and that the inhibition is related to Ca2+ and voltage-gated L- and T-type Ca2+ channels.

中文翻译：

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通过暴露于 7.83+/-0.3Hz 的方波抑制 B16F10 癌细胞生长涉及 L 型和 T 型钙通道

极低频电磁场 (ELF-EMF) 暴露会影响许多生物系统；这些影响主要与 ELF-EMF 的强度、持续时间、频率和模式有关。在这项研究中，暴露于 7.83±0.3 Hz（扫描步长 0.1 Hz）的方波显示可抑制 B16F10 黑色素瘤肿瘤细胞的生长。此外，磁场的分布由毕奥-萨伐尔定律计算并使用MATLAB绘制。体外研究表明，接触方波 48 小时后，B16F10 细胞增殖减少，Ca2+ 流入增加。Ca2+ 流入也被电压门控 L 型和 T 型 Ca2+ 通道的抑制部分阻止。