ABSTRACT

1. This work examined the effects of purified lycopene (LYC) supplementation or a source of LYC as tomato powder (TP) on productive performance, egg yolk cholesterol levels as well as gene expression related to mechanism and regulation of cholesterol.

2. One hundred and fifty laying hens (Lohman LSL, hybrid) were randomly divided into one of three treatments, with 10 replicates of five hens per cage, totalling 50 hens per treatment. The hens were fed either a standard diet (control) or a standard diet supplemented with 20 mg purified lycopene/kg diet (LYC) or an equal amount of lycopene-containing tomato powder (TP) for 12 weeks.

3. Feed consumption, egg production, and feed efficiency remained similar among treatments (P ≥ 0.27). Supplementing lycopene, either as a purified form or in TP, increased the levels of serum and egg yolk lycopene and reduced serum and egg yolk cholesterol concentrations (P < 0.001). Supplementation in either form decreased gene expression for intestinal NPC1L1, MTP, ACAT2, hepatic SREBP1c, ACLY, and LXRα but increased hepatic ABCG5 and ABCG8 (P < 0.001).

4. The results of the present work revealed that egg yolk cholesterol metabolism is regulated by the modulation of a group of genes, particularly with LYC supplementation.

摘要

1. 这项工作检查了纯化番茄红素 (LYC) 补充剂或作为番茄粉 (TP) 的 LYC 来源对生产性能、蛋黄胆固醇水平以及与胆固醇机制和调节相关的基因表达的影响。

2. 150只蛋鸡（Lohman LSL，杂交种）随机分为三个处理之一，每笼5只鸡10个重复，每个处理共50只鸡。给母鸡饲喂标准日粮（对照）或添加 20 mg 纯化番茄红素/kg 日粮 (LYC) 或等量含番茄红素的番茄粉 (TP) 的标准日粮 12 周。

3. 饲料消耗量、产蛋量和饲料效率在处理之间保持相似（P ≥ 0.27）。以纯化形式或以 TP 形式补充番茄红素可增加血清和蛋黄番茄红素的水平，并降低血清和蛋黄胆固醇浓度 (P < 0.001)。任何一种形式的补充剂都降低了肠道 NPC1L1、MTP、ACAT2、肝脏 SREBP1c、ACLY 和 LXRα 的基因表达，但增加了肝脏 ABCG5 和 ABCG8（P < 0.001）。

4. 本研究的结果表明，蛋黄胆固醇的代谢受一组基因的调节，特别是在补充 LYC 的情况下。