Abstract Bacillus subtilis HLZ-68 can produce l-amino acid oxidase (l-AAO), and dl-arginine can be degraded asymmetrically by suspending the wet bacterial biomass in the degradation liquid. By adding oxygen-vectors to the fermentation medium, the collected amount of wet bacterial biomass can be increased. Taking n-dodecane, n-hexadecane, oleic acid, paraffin and n-hexane as oxygen-vectors, the optimal oxygen-vector was 1.2% (v/v) oleic acid. The wet weight biomass increased by 66.83% and the activity of l-AAO in the fermentation broth increased by 38.88% compared with those before the addition of oxygen-vector. The standard sample dl-arginine was derivatized by phenyl isothiocyanate, and then subjected to high-performance liquid chromatography (HPLC), and the obtained peak area and arginine content were used as standard curves to measure the dl-arginine. The content of d-arginine and l-arginine in the initial degradation solution was 50% each, and the bacterial cells were added to the initial degradation solution of dl-arginine. After 21 h of reaction, l-arginine was completely degraded, leaving 47% of d-arginine. d-alanine was easily extracted from the reaction solution using cation-exchange resin, after centrifugation, decolourization, concentration and vacuum drying, and the chemical and optical purity of the extracted d-arginine were 92.68% and 97.46%, respectively.

中文翻译：

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枯草芽孢杆菌HLZ-68通过氧载体增强l-氨基酸氧化酶的产生和dl-精氨酸不对称降解为d-精氨酸

摘要 枯草芽孢杆菌HLZ-68可产生l-氨基酸氧化酶（l-AAO），dl-精氨酸可通过将湿的细菌生物质悬浮在降解液中进行不对称降解。通过向发酵培养基中添加氧载体，可以增加湿细菌生物质的收集量。以正十二烷、正十六烷、油酸、石蜡和正己烷为氧载体，最佳氧载体为1.2%（v/v）油酸。与添加氧载体前相比，湿重生物量增加了66.83%，发酵液中l-AAO的活性增加了38.88%。标准样品dl-精氨酸经异硫氰酸苯酯衍生，然后进行高效液相色谱（HPLC），并将得到的峰面积和精氨酸含量作为标准曲线测量dl-精氨酸。初始降解液中d-精氨酸和l-精氨酸含量各为50%，将菌体加入dl-精氨酸初始降解液中。反应 21 小时后，L-精氨酸完全降解，剩下 47% 的 d-精氨酸。d-丙氨酸易于用阳离子交换树脂从反应液中提取，经离心、脱色、浓缩、真空干燥后，提取的d-精氨酸的化学和光学纯度分别为92.68%和97.46%。l-精氨酸被完全降解，留下47%的d-精氨酸。d-丙氨酸易于用阳离子交换树脂从反应液中提取，经离心、脱色、浓缩和真空干燥后，提取的d-精氨酸的化学和光学纯度分别为92.68%和97.46%。l-精氨酸被完全降解，留下47%的d-精氨酸。d-丙氨酸易于用阳离子交换树脂从反应液中提取，经离心、脱色、浓缩和真空干燥后，提取的d-精氨酸的化学和光学纯度分别为92.68%和97.46%。