Abstract

Conventional cotton scouring in the textile industry using alkali results in huge environmental impact which can be overcome by using enzymes. Pectinase along with cutinase gives enhanced bioscouring results. Cutin was extracted from tomato peels and was used as substrate in the microbial media. The strain isolated from tomato peel was identified as Acinetobacter baumannii AU10 by 16S rDNA sequencing. The cutinase production was optimized by Placket-Burman and Response Surface Methodology (RSM) and the maximum production of 82.75 U/mL obtained at sucrose 6.68% (w/v), gelatin 2.74 g/L at a temperature of 35.93 °C. Cutinase was purified by ammonium sulfate precipitation, hydrophobic interaction chromatography and ion exchange chromatography with a recovery of 25.6% and specific activity of 38030 U/mg. The confirmation test for the purity of cutinase was analyzed by RP-HPLC. The molecular mass of cutinase was determined as 28.9 kDa by SDS-PAGE technique. Scanning electron microscopic analysis showed a rough and open primary wall surface on the cutinase bioscoured fabric which confirmed its activity on cutin present in the cotton fabric. Additionally, the cutinase-bioscoured samples showed better absorbency than the untreated samples. Therefore, enzymatic scouring increases wetting capacity of scoured cotton and also helps to reduce environmental pollution.

摘要

纺织工业中使用碱的常规棉花精练会导致巨大的环境影响，而这可以通过使用酶来克服。果胶酶与角质酶一起提供增强的生物煮练结果。从番茄皮中提取角质并用作微生物培养基中的底物。从番茄皮中分离得到的菌株被鉴定为鲍曼不动杆菌AU10通过 16S rDNA 测序。通过 Placket-Burman 和响应面方法 (RSM) 优化角质酶的产生，在 35.93 °C 的温度下，在蔗糖 6.68% (w/v)、明胶 2.74 g/L 下获得 82.75 U/mL 的最大产量。角质酶经硫酸铵沉淀、疏水作用层析和离子交换层析纯化，回收率为25.6%，比活为38030 U/mg。通过RP-HPLC分析角质酶纯度的确认试验。通过SDS-PAGE技术测定角质酶的分子量为28.9 kDa。扫描电子显微镜分析显示角质酶生物煮练织物上的粗糙和开放的初级壁表面证实了其对棉织物中存在的角质的活性。此外，角质酶生物精练的样品比未处理的样品表现出更好的吸收性。因此，酶法煮练提高了洗净棉的润湿能力，也有助于减少环境污染。