MADS-box transcription factors play crucial roles in regulating development processes and biosynthesis of secondary metabolites in eukaryotes. However, the role of MADS-box transcription factors vary among fungal species, and their function remains unclear in the medicinally and economically important fungus Ganoderma lucidum. In this study, we characterized a MADS-box gene, GlMADS1, in G. lucidum. Analyses using quantitative real-time polymerase chain reaction (qRT-PCR) showed that GlMADS1 expression levels were up-regulated from the mycelia to the primordia stage. In order to further evaluate the effect of MADS-box transcription factors on secondary metabolism, we utilized RNA interference (RNAi) to silence GlMADS1 in G. lucidum. Ganoderic acid (GA) and flavonoid contents were enhanced in GlMADS1-silenced strains, suggesting that GlMADS1 negatively regulates GA and flavonoid accumulation.

中文翻译：

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MADS-box 转录因子 GlMADS1 调控灵芝次生代谢

MADS-box 转录因子在调节真核生物次级代谢产物的发育过程和生物合成中起着至关重要的作用。然而，MADS-box 转录因子的作用因真菌物种而异，其在药用和经济上重要的真菌灵芝中的功能尚不清楚。在这项研究中，我们表征了灵芝中的 MADS-box 基因 GlMADS1。使用定量实时聚合酶链反应 (qRT-PCR) 进行的分析表明，GlMADS1 表达水平从菌丝体到原基阶段上调。为了进一步评估 MADS-box 转录因子对次级代谢的影响，我们利用 RNA 干扰 (RNAi) 使灵芝中的 GlMADS1 沉默。GlMADS1 沉默菌株中灵芝酸 (GA) 和类黄酮含量增加，