ABSTRACT

In a study that aimed to fingerprint the phototrophic community of the biodeteriorated walls of the Old Cathedral of Coimbra (UNESCO World Heritage Site), an unknown Nostoc/Komarekiella-like cyanobacterium was isolated. We employed a polyphasic approach based on morphological, ecological and phylogenetic analyses of the partial 16S and the whole 16S-23S ITS rRNA regions to characterize this organism. Our strains shared 16S rRNA gene sequence similarity of 99–100% with GenBank-assigned ‘Nostoc sp.’ sequences, and 98% similarity with the genera Goleter, Roholtiella, Aulosira and Komarekiella. Despite being morphologically similar to Komarekiella, phylogenetic analyses placed our strains in a separated genus-level clade, distant from Nostoc sensu stricto and with Komarekiella as the closest taxon. Considering all molecular, phylogenetic and ecological data, we here propose Parakomarekiella sesnandensis F.Soares, V.Ramos & A.Portugal, gen. et sp. nov.

摘要

在一项旨在对科英布拉旧大教堂（联合国教科文组织世界遗产地）生物恶化墙壁的光养群落进行指纹识别的研究中，分离出一种未知的Nostoc / Komarekiella样蓝细菌。我们采用基于部分 16S 和整个 16S-23S ITS rRNA 区域的形态学、生态学和系统发育分析的多相方法来表征这种生物。我们的菌株与 GenBank 指定的“ Nostoc sp.”具有 99-100% 的 16S rRNA 基因序列相似性。序列，并且与Goleter、Roholtiella、Aulosira和Komarekiella属有 98% 的相似性。尽管在形态上与Komarekiella相似，系统发育分析将我们的菌株置于分离的属级进化枝中，远离严格意义上的Nostoc，而Komarekiella作为最接近的分类单元。考虑到所有分子、系统发育和生态学数据，我们在这里提出Parakomarekiella sesnandensis F.Soares, V.Ramos & A.Portugal, gen. 等 sp. 十一月