Association analysis is an alternative to conventional, family-based methods for detecting the location of gene(s) or quantitative trait loci (QTLs), and provides relatively high resolution in terms of defining the genome position of a gene or QTL. Flavour is an essential quality characteristic of soymilk; however, soymilk contains volatile compounds unacceptable to consumers. One of main constituents in the volatiles of normal soymilk is 2-heptenal, which is thought to be a degradative oxidation product of polyunsaturated acids. In this study, a genome-wide association study using 24 651 single-nucleotide polymorphisms (SNPs) was performed to identify quantitative trait nucleotides (QTNs) controlling 2-heptenal content in soybean (Glycine max (L.) Merr.) seed from a natural population of 110 soybean germplasm accessions. We detected 62 significant QTNs located on 18 different chromosomes that are significantly associated with 2-heptenal content in soybean seed. Among these, 17 QTNs co-localised with QTLs previously found to be related to protein, oil and/or fatty acid content in soybean seed. We also identified some candidate genes involved in lipid metabolism. These findings further our understanding of the genetic basis of 2-heptenal content in soybean seed and the improvement of marker-assisted breeding efficiency, which will be important for breeding soybean cultivars with low 2-heptenal content.

关联分析是用于检测基因或定量性状基因座（QTL）位置的常规，基于家庭的方法的替代方法，并且在定义基因或QTL的基因组位置方面提供了相对较高的分辨率。风味是豆浆的基本品质特征。然而，豆浆含有消费者不可接受的挥发性化合物。正常豆浆挥发物的主要成分之一是2-庚醛，其被认为是多不饱和酸的降解性氧化产物。在这项研究中，进行使用24 651个单核苷酸多态性（SNPs）的全基因组关联研究，以确定数量性状核苷酸（QTNs）大豆控制-2-庚烯醛含量（大豆（L.）Merr。）种子，其自然种群为110种大豆种质。我们检测到位于18个不同染色体上的62个重要QTN，这些染色体与大豆种子中的2庚烯含量显着相关。其中，与先前发现的QTL共定位的17个QTN与大豆种子中的蛋白质，油和/或脂肪酸含量有关。我们还确定了一些参与脂质代谢的候选基因。这些发现进一步加深了我们对大豆种子中2庚烯含量的遗传基础以及标记辅助育种效率的提高的了解，这对于育种2庚烯含量低的大豆品种至关重要。