Cuticular waxes play important eco‐physiological roles in protecting plants against abiotic and biotic stresses and show high sensitivity to environmental changes. In order to clarify the responses of cuticular waxes on faba bean (Vicia faba L.) leaves to different light wavelengths, the phenotypic plasticity of cuticular waxes was analyzed when plants were subjected to white, red, yellow, blue, and purple light. Leaf samples from yellow, purple, and white lights were further analyzed, and candidate genes of wax biosynthesis were selected by RNA‐seq technology and transcriptome processing. Yellow light increased the total wax coverage and changed the crystal structure compared with leaves under white light. Light wavelengths changed the relative abundance of dominant primary alcohol from C₂₄ under white, yellow, and red lights to C₂₆ under blue and purple lights. In total, 100,194 unigenes were obtained, and 10 genes were annotated in wax biosynthesis pathway, including VLCFAs elongation (KCS1, KCS4, LACS2 and LACS9), acyl reduction pathway (FAR3 and WSD1), and decarboxylation pathway (CER1, CER3 and MAH1). qRT‐PCR analysis revealed that yellow and purple lights significantly influenced the expression levels of these genes. Yellow light also increased the water loss rate and decreased the photosynthesis rate. Light at different wavelengths particularly yellow light induced the changes of phenotypic plasticity of cuticular waxes, which thus altered the leaf eco‐physiological functions. The expression levels of genes related to wax biosynthesis were also altered by different light wavelengths, suggesting that light at different wavelengths may also be applied in selecting candidate genes involved in wax biosynthesis in other crops.

中文翻译：

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蚕豆表皮蜡对光波长的响应及表皮蜡生物合成候选基因的选择

表皮蜡在保护植物免受非生物和生物胁迫方面起着重要的生态生理作用，并且对环境变化表现出高度敏感性。为了阐明蚕豆（Vicia faba L.）叶片上的表皮蜡对不同光波长的响应，分析了当植物受到白色，红色，黄色，蓝色和紫色光照射时表皮蜡的表型可塑性。进一步分析了黄，紫和白光下的叶片样品，并通过RNA-seq技术和转录组加工技术选择了蜡生物合成的候选基因。与白光下的叶子相比，黄光增加了蜡的总覆盖率并改变了晶体结构。光波长改变了C ₂₄中主要伯醇的相对丰度在C，C _26的白，黄，红灯下在蓝色和紫色的灯光下。总共获得了100,194个单基因，并在蜡生物合成途径中注释了10个基因，包括VLCFA延伸（KCS1，KCS4，LACS2和LACS9），酰基还原途径（FAR3和WSD1）和脱羧途径（CER1，CER3和MAH1） 。qRT-PCR分析显示黄色和紫色的光显着影响这些基因的表达水平。黄光还增加了失水率并降低了光合作用率。不同波长的光，尤其是黄光，会引起表皮蜡表型可塑性的变化，从而改变了叶片的生态生理功能。与蜡生物合成相关的基因的表达水平也因不同的光波长而改变，