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Telecentric design for digital‐scanning‐based HiLo optical sectioning endomicroscopy with an electrically tunable lens
Journal of Biophotonics ( IF 2.8 ) Pub Date : 2020-10-30 , DOI: 10.1002/jbio.202000335
Haw Hsiao, Chen‐Yen Lin, Sunil Vyas, Kuang‐Yuh Huang, J. Andrew Yeh, Yuan Luo

Confocal endoscopy has been widely used to obtain fine optically sectioned images. However, confocal endomicroscopic images are formed by point‐by‐point scanning in both lateral and axial directions, which results in long image acquisition time. Here, an endomicroscope with telecentric configuration is presented to achieve nonmechanical and rapid axial scanning for volumetric fluorescence imaging. In our system, optical sectioning in wide‐field fashion is obtained through HiLo imaging with a digital micromirror device. Axial scanning, without mechanical moving parts, is conducted by digital focus adjustment using an electrically tunable lens, offering constant magnification and contrast. We demonstrate imaging performance of our system with optically sectioned images using fluorescently labeled beads, as well as ex vivo mice cardiac tissue samples. Our system provides multiple advantages, in terms of improved scanning range, and reduced image acquisition time, which shows great potentials for three‐dimensional biopsies of volumetric biological samples.image

中文翻译:

基于数字扫描的HiLo光学切片内窥镜的远心设计,带电子可调透镜

共焦内窥镜已被广泛用于获得精细的光学切片图像。但是,共焦内窥镜图像是通过在横向和轴向上逐点扫描形成的,因此需要较长的图像采集时间。在这里,提出了具有远心配置的内窥镜,以实现用于体积荧光成像的非机械和快速轴向扫描。在我们的系统中,通过使用数字微镜设备进行的HiLo成像可以获得广角镜的光学切片。轴向扫描,不带机械移动部件,是通过使用电子可调透镜通过数字焦点调节进行的,从而提供恒定的放大率和对比度。我们使用荧光标记的珠子以及离体小鼠心脏组织样本通过光学切片图像展示了我们系统的成像性能。图像
更新日期:2020-10-30
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