Porphyromonas gingivalis is a Gram‐negative anaerobic pathogen found in subgingival plaque associated with progressive periodontitis. Proteins associated with the outer membrane (OM) of Gram‐negative pathogens are particularly important for understanding virulence and for developing vaccines. The aim of this study was to establish a reliable list of outer membrane associated proteins (Omps) for this organism. Starting with a list of 99 experimentally determined Omps, several bioinformatics tools were used to predict a further 52 proteins, leading to a predicted OM proteome of 151 proteins. The tools used included databases of protein families, prediction of OM β‐barrels and structural homology. The list includes 33 T9SS cargo proteins, 43 lipoproteins and 66 OM β‐barrel proteins with some overlap between categories. The proteins are discussed both in these structural categories as well as their various functions in OM biogenesis, nutrient acquisition, protein secretion, adhesion and efflux. Proteins that were previously shown to be part of large complexes are highlighted and cross reference is provided to a previous major study of protein localization in P. gingivalis. Finally, proteins were also scored according to their level of conservation within the Bacteroidales taxon. Low scores were shown to correlate with virulence factors and may be predictive of novel virulence factors.

中文翻译：

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定义牙龈卟啉单胞菌的外膜蛋白质组

牙龈卟啉单胞菌是革兰氏阴性厌氧菌病原，存在于与进行性牙周炎相关的龈下斑块中。与革兰氏阴性病原体的外膜（OM）相关的蛋白质对于理解毒力和开发疫苗尤为重要。这项研究的目的是为该生物建立可靠的外膜相关蛋白（Omps）清单。从99个实验确定的Omps列表开始，使用了多种生物信息学工具来预测另外52种蛋白质，从而预测出151种蛋白质的OM蛋白质组。使用的工具包括蛋白质家族数据库，OMβ-桶的预测和结构同源性。该列表包括33种T9SS货物蛋白，43种脂蛋白和66种OMβ-桶蛋白，类别之间有些重叠。在这些结构类别中讨论了蛋白质，并讨论了它们在OM生物发生，营养获取，蛋白质分泌，粘附和外排中的各种功能。突出显示了以前显示为大复合物一部分的蛋白质，并将交叉参考提供给先前对蛋白质局部定位的主要研究。牙龈卟啉单胞菌。最后，还根据细菌杆菌分类中的保守程度对蛋白质进行了评分。低分显示出与毒力因子相关，可能预示了新的毒力因子。