Coffee is rich in antioxidant and has been shown to confer various health benefits. Here, we investigated the effect of single‐dose coffee consumption in healthy human subjects. About 30 healthy volunteers were recruited and given a serving of sugar free black coffee. Urine and fecal samples were collected and analyzed. Significant changes in urinary metabolites relating to coffee, gut microbial and host energy metabolisms were observed post‐coffee consumption. Clear sex differences were also observed in the urinary metabolic profiles pre‐ and post‐coffee consumption. Sex differences in richness and composition of gut microbiota were observed, however, the effect of single‐dose coffee consumption on host gut microbiota were unremarkable. These findings indicated that single‐dose coffee consumption affects sex‐specific host metabolic responses that relates to gut‐microbe and energy metabolism. This study demonstrated the utility of systems biology tools to unravel complexity of host‐diet biology and gut microbial responses.

中文翻译：

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喝咖啡揭示健康成年人宿主内源性代谢和肠道菌群的性别差异

咖啡富含抗氧化剂，已被证明具有多种健康益处。在这里，我们研究了单剂量咖啡摄入对健康人的影响。招募了约30名健康志愿者，并提供了无糖黑咖啡。收集尿液和粪便样品并进行分析。饮用咖啡后，观察到与咖啡，肠道微生物和宿主能量代谢有关的尿代谢物发生了显着变化。在咖啡摄入前后的尿代谢状况中也观察到明显的性别差异。观察到肠道菌群的丰富度和组成存在性别差异，但是单剂量咖啡摄入对宿主肠道菌群的影响并不显着。这些发现表明，单剂量咖啡的摄入会影响与肠道微生物和能量代谢有关的性别特定宿主代谢反应。这项研究证明了系统生物学工具可以揭示宿主饮食生物学和肠道微生物反应的复杂性。