Phthalates and bisphenol A interfere with the synthesis, secretion, transport, binding, metabolism, and excretion of endogenous hormones and, for this reason, are classified as endocrine disruptors.

We are here presenting an analytical method for the simultaneous detection of six phthalates metabolites and bisphenol A in different biological fluids (urine, serum and follifular fluid) by liquid chromatography coupled to tandem mass spectrometry. The quantification was carried out in negative electrospray ionization mode using selected reaction monitoring as acquisition mode. Different extraction protocols, using either solid phase or liquid/liquid extraction, were comparatively evaluated to optimize the sample preparation procedure. Solid-phase extraction was chosen as it ensured the best recovery and overall sensitivity. The method was successfully validated: recovery varying in the range 71 ± 2%–107 ± 6% depending on the target analyte and the matrix considered, intra-assay and inter-assay precision ≤ 12% for follicular fluid, ≤11% for serum and ≤ 10% for urine and accuracy ≤ 115% for follicular fluid, ≤113% for serum ≤ 115% for urine , linearity with R² > 0.99, with the exception of MEP (recovery 64 ± 8%, intra-assay precision ≤ 20%, inter-assay precision ≤ 16% for follicular fluid). The actual applicability of the method developed and validated in this study was assessed by the analysis of real samples, including 10 specimens of follicular fluid, serum and urine samples, that showed the presence of phthalates metabolites and Bisphenol A, and confirming that the newly developed method can be applied in the routine clinical laboratory for the identification and quantitation of these endocrine-disrupting chemicals.

邻苯二甲酸盐和双酚 A 会干扰内源性激素的合成、分泌、运输、结合、代谢和排泄，因此被归类为内分泌干扰物。

我们在此介绍了一种通过液相色谱与串联质谱联用同时检测不同生物体液（尿液、血清和卵泡液）中的六种邻苯二甲酸酯代谢物和双酚 A 的分析方法。定量是在负电喷雾电离模式下进行的，使用选择的反应监测作为采集模式。使用固相或液/液萃取的不同萃取方案进行了比较评估，以优化样品制备程序。选择固相萃取是因为它确保了最佳的回收率和整体灵敏度。该方法得到了成功验证：回收率在 71 ± 2%–107 ± 6% 的范围内变化，具体取决于目标分析物和所考虑的基质、分析内和 卵泡液的批间精密度≤12%，血清≤11%，尿液≤10%，准确度≤115%，血清≤113%，尿液≤115%，线性R ²  > 0.99，有MEP 除外（回收率 64 ± 8%，测定内精密度 ≤ 20%，卵泡液测定间精密度 ≤ 16%）。在本研究中开发和验证的方法的实际适用性是通过对真实样品的分析来评估的，包括 10 个卵泡液、血清和尿液样本，显示存在邻苯二甲酸酯代谢物和双酚 A，并确认新开发的该方法可用于常规临床实验室对这些干扰内分泌的化学物质进行鉴定和定量。