Background

2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyrimidine (PhIP), one of the most abundant heterocyclic aromatic amines (HAA) formed by cooking meat at high temperatures, may modify humans and rodents through the metabolic process prior to affecting nervous system development. In humans and rodents may be modified by metabolic processes and then affecting nervous system development.

Methods

In this paper, PhIP was used to prepare a chicken embryo model with abnormal embryonic nervous system defects. Sulforaphane (SFN) is a derivative of a glucosinolate, which is abundant in cruciferous vegetables, and can pass through the placental barrier. Moreover, SFN has antioxidant and anti-apoptotic functions and is considered as a bioactive antioxidant with significant neuroprotective effects. Nano-sulforaphane (Nano-SFN, sulforaphane nanoparticles) was prepared by self-assembly using biocompatible, biodegradable methoxy polyethylene glycol 5000-b-polyglutamic acid 10,000 (mPEG5K-PGA10K) as the substrate, to explore the new application of Nano-SFN and its modified compounds as leading compounds in protecting against the abnormal development of the embryonic nervous system.

Results

The results show that Nano-SFN could protect against PhIP-induced central nervous system (CNS, derived from neural tube) and peripheral nervous system (PNS, derived from neural crest cells, NCCs) defects and neural tube defects (NTDs), and increase the embryo survival rate.

Conclusions

This study indicates that Nano-SFN can effectively alleviate the developmental defects of embryonic nervous system induced by PhIP in the microenvironment and has a protective effect on embryonic development. It not only helps with expanding the application of SFN and improving its medicinal value, but also provides a possibility of SFN being developed as a novel drug for neuroprotection.

中文翻译：

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纳米萝卜硫素减弱 PhIP 诱导的早期异常胚胎神经发育

背景

2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyrimidine (PhIP) 是最丰富的杂环芳香胺 (HAA) 之一，由高温烹饪肉类形成，可通过代谢改变人类和啮齿动物影响神经系统发育之前的过程。在人类和啮齿动物中，代谢过程可能会改变，然后影响神经系统的发育。

方法

本文利用PhIP制备了胚胎神经系统异常缺陷的鸡胚胎模型。Sulforaphane (SFN) 是芥子油苷的衍生物，在十字花科蔬菜中含量丰富，可以通过胎盘屏障。此外，SFN具有抗氧化和抗凋亡功能，被认为是具有显着神经保护作用的生物活性抗氧化剂。以生物相容性、可生物降解的甲氧基聚乙二醇5000-b-聚谷氨酸10000（mPEG5K-PGA10K）为底物，通过自组装制备纳米萝卜硫素（Nano-SFN，萝卜硫素纳米粒），探索纳米SFN和其修饰的化合物是防止胚胎神经系统异常发育的主要化合物。

结果

结果表明，Nano-SFN 可以预防 PhIP 诱导的中枢神经系统（CNS，来自神经管）和周围神经系统（PNS，来自神经嵴细胞，NCCs）缺陷和神经管缺陷（NTDs），并增加胚胎存活率。

结论

本研究表明，Nano-SFN可有效缓解微环境中PhIP诱导的胚胎神经系统发育缺陷，对胚胎发育具有保护作用。它不仅有助于扩大SFN的应用范围，提高其药用价值，而且为将SFN开发为神经保护新药提供了可能。