Cell plasma membrane, the boundary between an individual live cell and its microenvironment, plays important roles in cellular communication and signaling, and associates with many physiological and pathological processes. For better understanding the membrane-related biological processes, a number of fluorescent probes have been developed in the past few years. According to their different functions and targeting mechanisms, we herein provide an overview of the advances in the development of fluorescent probes for cell plasma membrane biology and biophysics research. Fluorescent probes specific for cell plasma membrane labelling are initially discussed based on their different targeting mechanisms, which is followed by the summary of membrane-targeting responsive fluorescence probes for various biomolecules detection. Challenges and future research directions for the development of new generation fluorescent probes for cell plasma membrane dynamics investigations are outlined in the end.

细胞质膜是单个活细胞与其微环境之间的边界，在细胞通讯和信号传导中起着重要作用，并与许多生理和病理过程相关。为了更好地了解膜相关的生物学过程，在过去几年中开发了许多荧光探针。根据它们的不同功能和靶向机制，我们在此概述了用于细胞质膜生物学和生物物理学研究的荧光探针的开发进展。首先基于它们不同的靶向机制讨论了特异性针对细胞质膜标记的荧光探针，然后概述了用于各种生物分子检测的膜靶向响应性荧光探针。