P. aeruginosa causes mostly both community-acquired and nosocomial infections, which leads to serious therapeutic challenges for treatment and requirement of appropriate therapeutic agent is needed which can combat antibiotic resistance. The research work was performed to investigate the effect of Zinc Oxide nanoparticles (ZnO NPs) in combination with Meropenem, Ciprofloxacin, and Colistin against clinical isolated strains of P. aeruginosa and ATCC 27853 strain.

The minimum inhibitory concentration (MIC) of ZnO NPs and the antibiotics (Meropenem, Ciprofloxacin, and Colistin), was determined by the microdilution method and the results of MIC values were ranging between 1 to 16 µg/mL was found to be shown for antibiotics and ZnO NPs found to showed highest MIC values ranging from 2000-4000 µg/mL. The fractional inhibitory concentration index (FICI) was calculated using checkerboard method to test the combinations of ZnO NPs and the antibiotics (Meropenem, Ciprofloxacin, and Colistin), and among all the six P.aeruginosa clinical isolated strains P.aeruginosa (MRO-16-3 and MRO-16-4), showed FICI as 0.24 and 0.39 9, whereas P.aeruginosa ATCC 27853 strain showed FICI as 0.41 which indicates synergistic effect with Colistin.

The time kill growth curve showed synergistic effect for the combination of Colistin and ZnO NPs against P. aeruginosa (MRO-16-3 and MRO-16-) strains. P. aeruginosa (MRO-16-3) was found to be highly sensitive to Colistin with an MIC of 2 µg/mL, which has shown to reduced bacterial growth to zero colonies after 24 hours of incubation.

In conclusion, combination of Colistin and ZnO NPs at appropriate dosage intervals might be beneficial as using therapeutic agent in treatment of P. aeruginosa ailments.

铜绿假单胞菌主要引起社区获得性和医院感染，这导致严重的治疗挑战，需要合适的治疗剂来对抗抗生素耐药性。进行研究工作以研究氧化锌纳米颗粒（ZnO NPs）与美罗培南，环丙沙星和考力汀联合对临床分离的铜绿假单胞菌和ATCC 27853菌株的影响。

微量稀释法确定了ZnO NP和抗生素（美洛培南，环丙沙星和科力汀）的最小抑菌浓度（MIC），结果表明，MIC值在1至16 µg / mL之间ZnO NPs的MIC值最高，为2000-4000 µg / mL。分数抑制浓度指数（FICI）使用棋盘方法测试的ZnO纳米颗粒和抗生素（美罗培南，环丙沙星，和粘菌素）的组合计算出的，并在所有的六个绿脓杆菌临床分离株绿脓杆菌（MRO-16 -3和MRO-16-4）的FICI为0.24和0.39 9，而铜绿假单胞菌 ATCC 27853菌株显示FICI为0.41，表明与Colistin有协同作用。

时间杀灭生长曲线显示了针对铜绿假单胞菌（MRO-16-3和MRO-16-）菌株的Colistin和ZnO NP的协同作用。发现铜绿假单胞菌（MRO-16-3）对Colistin高度敏感，MIC为2 µg / mL，已显示在培养24小时后可将细菌生长减少至零菌落。

总之，以适当的剂量间隔联合应用Colistin和ZnO NP作为治疗铜绿假单胞菌病的治疗剂可能是有益的。