Novel approaches are obligatory to treat chronic intracellular bacterial infectious diseases like Brucellosis specifically, are very complicated to deal with. The aim of the study to take upon nanotechnology approach to exploit the efficacy of the synthesized nanoparticles, to overcome barriers for treatment of Brucella species and other pathogens. Present study used Rivina humilis extract as reductant of silver ions for synthesis of silver nanoparticles for the first time. Rh-AgNP’s was characterized by UV–visible spectroscopy, DLS, FT-IR, SEM, EDS, TEM and XRD. Radical scavenging, antibrucellosis, bactericidal activity was evaluated. Clinical application was assessed by Rate of haemolysis, fibrinolytic and Hemagglutination activity. UV–visible spectrum of synthesized Rh-AgNP’s showed maximum peak at 440 nm indicating the formation of nanoparticles. TEM showed that the average particle size of nanoparticles 51 nm with spherical shape, DLS depicted monodisperse state in water; EDS confirmed the presence of silver metal. Rh-AgNP’s exhibited potential antibrucellosis activity against B. abortus, B. melitensis and B. suis effective inhibition at 800 μg/mL. The bio-compatibility of Rh-AgNP’s was established by rate of haemolysis, hemagglutination and fibrinolytic activity. For the first time it has been proved that Rh-AgNP’s have efficacy as antimicrobial agent with potential application in the biological domain.

特别地，必须要有新颖的方法来治疗慢性细胞内细菌感染性疾病，如布鲁氏菌病。该研究的目的是采用纳米技术方法来开发合成纳米颗粒的功效，克服治疗布鲁氏菌物种和其他病原体的障碍。本研究使用Rivina humilis提取物作为银离子的还原剂，首次用于银纳米颗粒的合成。Rh-AgNP的特征在于紫外可见光谱，DLS，FT-IR，SEM，EDS，TEM和XRD。评估了自由基清除，抗布鲁氏菌病，杀菌活性。通过溶血率，纤溶和血凝活性评估临床应用。合成的Rh-AgNP的紫外可见光谱在440 nm处显示最大峰，表明形成了纳米颗粒。透射电镜（TEM）表明，球形纳米颗粒的平均粒径为51nm，DLS在水中呈单分散态。EDS证实存在银金属。Rh-AgNP's对流产双歧杆菌，melitensis和猪双歧杆菌具有潜在的抗布鲁氏菌病活性在800μg/ mL时有效抑制。Rh-AgNP的生物相容性是由溶血，血凝和纤溶活性确定的。首次证明，Rh-AgNP具有作为抗菌剂的功效，并可能在生物学领域得到应用。