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Spatial and temporal changes in follicle distribution in the human ovarian cortex
Reproductive BioMedicine Online ( IF 4 ) Pub Date : 2020-10-25 , DOI: 10.1016/j.rbmo.2020.10.013
Annejet Schenck 1 , Maria Vera-Rodriguez 2 , Gareth Greggains 2 , Ben Davidson 3 , Péter Fedorcsák 4
Affiliation  

Research question

How does follicle distribution evolve in the human ovarian cortex between the ages of 20 and 35 years?

Design

Fragments of ovarian cortex from women undergoing unilateral oophorectomy for fertility preservation were obtained for quantitative histological assessment, including recording the two-dimensional coordinates of the follicles. Data were analysed using spatial statistical methods.

Results

A total of 53 ovarian cortex tissue samples, containing 1–803 follicles each, were obtained from 14 women aged 20–35 years. Primordial and transitory follicles lay in a clustered manner in the human ovarian cortex, with an average cluster radius of around 270 µm (95% confidence interval 154–377 µm; n = 49). Follicle density declined with age (P = 0.006, n = 13), and the distance from the nearest neighbouring follicle increased (P = 0.004, n = 13). Cluster radius decreased with age (P = 0.02, n = 13), but the degree of clustering tended to increase (P = 0.11, n = 13). In the majority of the samples, follicles at different stages lay in different clusters (P < 0.05, n = 13).

Conclusions

This study shows that primordial and transitory follicles lie in different clusters in the human ovarian cortex. Spatio-temporal computer simulation suggests that interfollicular signals may hinder follicle loss and may therefore drive clustered follicle distribution. In clinical practice, the woman's age should be taken into account when assessing follicle density, as follicle distribution is increasingly clustered with advancing age.



中文翻译:

人卵巢皮质卵泡分布的时空变化

研究问题

20 至 35 岁之间的人类卵巢皮质中的卵泡分布如何演变?

设计

从接受单侧卵巢切除术以保留生育能力的妇女的卵巢皮质碎片进行定量组织学评估,包括记录卵泡的二维坐标。使用空间统计方法分析数据。

结果

从 14 名 20-35 岁的女性中获得了总共 53 个卵巢皮质组织样本,每个样本包含 1-803 个卵泡。原始和暂时性卵泡在人类卵巢皮质中呈簇状分布,平均簇半径约为 270 µm(95% 置信区间 154–377 µm;n  = 49)。卵泡密度随着年龄的增长而下降(P  =0.006,n  =13),与最近的相邻卵泡的距离增加(P  =0.004,n  =13)。聚类半径随着年龄的增长而减小(P  = 0.02, n  = 13),但聚类程度有增加的趋势(P  = 0.11, n = 13)。在大多数样本中,不同阶段的卵泡位于不同的簇中(P  < 0.05,n  = 13)。

结论

这项研究表明,原始卵泡和暂时性卵泡位于人类卵巢皮质的不同簇中。时空计算机模拟表明,卵泡间信号可能会阻碍卵泡丢失,因此可能会驱动成簇的卵泡分布。在临床实践中,评估卵泡密度时应考虑女性的年龄,因为随着年龄的增长,卵泡分布越来越集中。

更新日期:2020-10-25
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