Abstract This work is dedicated to the synthesis and characterization of antibody labeled magnetic nanoparticles as promising tool for biomedical applications. Single core magnetic nanoparticles were synthesized by thermal decomposition technique, followed by surface modification with ligand based on polyethylene glycol derivative. Fluorochrome-labeled species-specific antibodies were covalently conjugated to MNPs through a polyethylene glycol linker. The immunofluorescent analysis showed the successful binding of anti-α-tubulin or anti-β-catenin antibodies conjugated MNPs to α-tubulin and β-catenin in 4T1 cells of mouse breast cancer.

中文翻译：

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用于靶向递送至细胞膜的抗体标记的单核磁性纳米粒子的合成和深入分析

摘要 这项工作致力于合成和表征抗体标记的磁性纳米粒子，作为生物医学应用的有前途的工具。通过热分解技术合成单核磁性纳米粒子，然后用基于聚乙二醇衍生物的配体进行表面改性。荧光染料标记的物种特异性抗体通过聚乙二醇接头与 MNP 共价结合。免疫荧光分析表明，在小鼠乳腺癌的 4T1 细胞中，抗​​ α-微管蛋白或抗 β-连环蛋白抗体与 MNPs 成功结合到 α-微管蛋白和 β-连环蛋白。