Bacillus licheniformis is a major source of microbial contamination to dairy industry, and biofilm formation by this spoilage bacterium aggravates the safety issues. Especially for milk powder manufactures, the evaporation process at temperatures between 50 °C and 70 °C before spray drying, is a critical control point against thermophilic bacteria multiplication. In our study, metabolomics analysis was performed to investigate dynamic changes of the metabolites and their roles during process of biofilm development of B. licheniformis at 55 °C for 24 h. Amino acid metabolism was quite active, with cooperation from lipid metabolism, carbohydrate metabolism and nucleotide metabolism. Amino acid biosynthesis provided significant contributions especially during early biofilm development from 8 to 12 h. Metabolites involved in specific pathways of arginine biosynthetic, galactose metabolism and sphingolipid metabolism played a crucial role in building biofilm. This work provided new insights into dynamic metabolic alternations and a comprehensive network during B. licheniformis biofilm development, which will extend the knowledge on the metabolic process of biofilm formation by B. licheniformis. The results are helpful in creating better environmental hygiene in dairy processing and new strategies for ensuring quality of dairy products.

地衣芽孢杆菌是乳制品业微生物污染的主要来源，这种腐败细菌形成生物膜加剧了安全问题。特别是对于奶粉制造商而言，喷雾干燥之前在50°C至70°C之间的温度进行蒸发过程是防止嗜热细菌繁殖的关键控制点。在我们的研究中，进行了代谢组学分析，以研究代谢产物的动态变化及其在地衣芽孢杆菌生物膜发育过程中的作用。在55°C下放置24小时。氨基酸代谢非常活跃，脂质代谢，碳水化合物代谢和核苷酸代谢共同作用。氨基酸的生物合成提供了重要的贡献，特别是在从8到12小时的早期生物膜形成过程中。涉及精氨酸生物合成，半乳糖代谢和鞘脂代谢特定途径的代谢物在构建生物膜中起着至关重要的作用。这项工作为地衣芽孢杆菌生物膜形成过程中的动态代谢变化和一个全面的网络提供了新见解，这将扩展关于地衣芽孢杆菌生物膜形成代谢过程的知识。。这些结果有助于在乳制品加工过程中创造更好的环境卫生状况，并有助于确保乳制品质量的新策略。