Salmonella enterica serovar Enteritidis (S. Enteritidis) presents an excellent capacity to survive in egg white, which is a hostile environment for bacterial growth. To reveal its survival mechanism, this study focuses on the specific gene SEN1393, which has been found to exist only in the genomic sequence of S. Enteritidis. The survival capacity of the deletion mutant strain ΔSEN1393 was proven to be significantly reduced after incubation in egg white. RNA sequencing and RT-qPCR results demonstrate that the expression levels of 19 genes were up-regulated, while the expression levels of 9 genes were down-regulated in egg white. These genes were classified into 6 groups based on their functional categories, namely the sulfate assimilation pathway, arginine biosynthesis, the tricarboxylic acid cycle, the fimbrial protein, the transport and chelation of metal ion, and others (sctT, rhs, and pspG). The strain ΔSEN1393 was deduced to damage FeS cluster enzymes and increase the sulfate and iron requirements, and to reduce bacterial motility and copper homeostasis. Via InterProScan analysis, the gene SEN1393 was speculated to encode a TerB-like and/or DjlA-like protein, and therefore, together with cysJ, possibly reduced the oxidative toxicities resulting from oxyanions such as tellurite, and/or improved CysPUWA conformation to restrain the uptake of the toxic oxyanions. In summary, the gene SEN1393 enabled the higher survival of S. Enteritidis in egg white as compared to other pathogens by regulating the sulfate assimilation pathway.

沙门氏菌血清型肠炎（小号。肠炎）呈现在蛋清，这是细菌生长的恶劣环境中生存的能力优异。为了揭示其存活机制，本研究关注于特定基因SEN1393，该基因仅存在于S的基因组序列中。。肠炎。在蛋白中孵育后，已证明缺失突变株ΔSEN1393的存活能力显着降低。RNA测序和RT-qPCR结果表明，蛋白中19个基因的表达水平上调，而9个基因的表达水平下调。这些基因根据其功能类别分为6类，即硫酸盐同化途径，精氨酸生物合成，三羧酸循环，纤维蛋白，金属离子的运输和螯合等（sctT，rhs和pspG）。推导应变ΔSEN1393破坏Fe硫使酶群集化，并增加了硫酸盐和铁的需求量，并降低了细菌的运动能力和铜稳态。通过InterProScan分析，推测SEN1393基因编码一个TerB样和/或DjlA样蛋白，因此，与cysJ一起，可能降低了由含氧阴离子（如亚碲酸盐）引起的氧化毒性，和/或改善了CysPUWA构象以抑制吸收有毒的氧阴离子。总而言之，基因SEN1393使S的存活率更高。通过调节硫酸盐同化途径，与其他病原体相比，蛋清中的肠炎。