Plant cell tissue and organ culture techniques provide an efficient way for the biosynthesis of high-value secondary metabolites in which elicitation has been recognized to be a potent approach in enriching the bioactive compounds. In this study, an effort was made to assess the influence of Cadmium chloride (CdCl₂) on alliin yield of different in vitro grown tissues such as callus, somatic embryos, leaves and roots of Allium sativum. The tissues were cultured in MS amended with various levels of CdCl₂ [control (0 mM), T1 (0.05 mM), T2 (0.1 mM), T3 (0.15 mM) and T4 (0.2 mM)] and the yield of alliin was quantified by using high performance thin layer chromatography (HPTLC). Biochemical analyses revealed that sample tissues under increasing CdCl₂ conditions accumulated higher levels of sugar, protein and proline with maximum content of sugar (19.61 mg g⁻¹ FW), protein (6.01 mg g⁻¹ FW) and proline (12.19 mg g⁻¹ FW) in leaves at T3 after 4 days of elicitor treatment. Changes within the activities of varied antioxidant enzymes like catalase (CAT), superoxide dismutase (SOD) and ascorbate peroxidase (APX) was assayed as the addition of CdCl₂ in the medium causes stress. We noted that with an elevated level of CdCl₂, antioxidant enzyme activity increased linearly with the highest activity observed in leaves at T4 of 6 days of elicitation (4.97 EU min⁻¹ mg⁻¹ protein CAT, 6.30 EU min⁻¹ mg⁻¹ protein SOD and 1.125 EU min⁻¹ mg⁻¹ protein APX). Increased activities of enzymes are involved in negating the cellular stress triggered due to the overproduction of ROS. The yield of alliin in response to varying levels of CdCl₂ was estimated by using a simple, rapid, and sensitive HPTLC method. The best separation was achieved using butanol:propanol:acetic acid:water (3:1:1:1) as mobile phase. For analysis of alliin, the plates were scanned at 550 nm after spraying with ninhydrin and a good resolution was achieved with Rf value of 0.66. In our study, the alliin yield enhanced in all tested cultures with the elicitor level up to T3. In leaves, maximum accumulation of alliin (3.37 μg g⁻¹ dry wt.) was noted on 0.15 mM (T3) CdCl₂ added medium. Our data indicated the increase of alliin in CdCl₂ added cultures, which suggests that the use of elicitors as a potential strategy for augmenting secondary metabolite synthesis.

植物细胞组织和器官培养技术为高价值次级代谢产物的生物合成提供了一种有效的方法，其中诱导被认为是富集生物活性化合物的有效方法。在这项研究中，努力评估氯化镉（CdCl ₂）对不同体外生长的组织，如愈伤组织，体细胞胚，大蒜和根的蒜素产量的影响。在用不同水平的CdCl ₂修正的MS中培养组织[对照（0mM），T1（0.05mM），T2（0.1mM），T3（0.15mM）和T4（0.2mM）]和蒜氨酸的产率通过使用高效薄层色谱法（HPTLC）定量。生化分析显示下增加的CdCl该样本组织₂条件积累更高水平的糖，蛋白质和脯氨酸的糖的最大含量（19.61毫克克^-1 FW），蛋白质（6.01毫克克^-1 FW）和脯氨酸（12.19毫克克^-诱导剂处理4天后，在T3的叶片中加入¹ FW）。添加CdCl _2可测定过氧化氢酶（CAT），超氧化物歧化酶（SOD）和抗坏血酸过氧化物酶（APX）等各种抗氧化酶的活性变化。在介质中会引起压力。我们注意到，随着CdCl ₂含量的升高，抗氧化酶的活性随着诱导6天的T4叶片中的最高活性而线性增加（4.97 EU min ^-1 mg ^-1蛋白CAT，6.30 EU min ^-1 mg ^-1蛋白SOD和1.125 EU min ^-1 mg ^-1蛋白APX）。酶活性的提高与否定由于ROS过量产生而引发的细胞应激有关。CdCl ₂水平变化时蒜氨酸的产量通过使用简单，快速且灵敏的HPTLC方法进行估算。使用丁醇：丙醇：乙酸：水（3：1：1：1）作为流动相可实现最佳分离。为了分析蒜氨酸，在用茚三酮喷洒后在550 nm扫描平板，并获得了良好的分离度，Rf值为0.66。在我们的研究中，诱导子水平达到T3时，所有测试培养物中的蒜氨酸产量均得到提高。在叶片中，在添加了0.15 mM（T3）CdCl _2的培养基上发现了蒜氨酸的最大积累量（3.37μgg ^-1干重）。我们的数据表明添加CdCl _2的培养物中蒜氨酸的增加，这表明使用激发子作为增加次生代谢产物合成的潜在策略。