Staphylococcus aureus is one of the leading causative of numerous infections that is resistant to various antibiotics. Since that we are facing with a serious problem of bacterial resistance it is necessary to find a new antibacterial agents in fight with it. Frangula alnus is traditionally used plant but its antibiofilm potential is poorly investigated. The aim of the study was to investigate antibiofilm activity of F. alnus ethyl-acetate extract (FA) toward S. aureus ATCC strains and clinical isolates as well as its influence on respiration in planktonic and biofilm form. The qualitative GCxGC–MS and quantitative LC–MS/MS analysis revealed that FA extract was rich in phenols and flavonoids and emodin, chatechin, and ester 4-ethoxy benzoic acid were the most dominant components. Results obtained through microdillution assay showed that FA possesses strong antibacterial activity. Furthermore, crystal violet staining of biofilm biomass demonstrated that extract had strong effect on biofilm formation of all tested strains while effect on preformed biofilms was less pronounced. The effect on biofilm was confirmed with scanning electron microscopy where the changes in biofilm structure were noticed. The activity of extract on the consumption of O₂ and production of CO₂ was monitored using the Micro-Oxymax respirometer. Interestingly, respiration of the most strains was decreased in planktonic form as well as in biofilms. Results obtained in this study are a good basis for further research in order to discover the mechanism of action of the FA extract on connection between biofilm and respiration.

金黄色葡萄球菌是对多种抗生素具有抵抗力的众多感染的主要病因之一。由于我们面临着严重的细菌耐药性问题，因此有必要寻找一种与之抗衡的新型抗菌剂。弗兰果（Frangula alnus）是传统上使用的植物，但其抗生物膜的潜力尚未得到充分研究。该研究的目的是调查的抗生物膜活性F.桤的乙酸乙酯提取物（FA）朝向金黄色葡萄球菌ATCC菌株和临床分离株及其对浮游生物膜和生物膜形式呼吸的影响。GCxGC-MS的定性分析和LC-MS / MS的定量分析表明，FA提取物中富含酚和类黄酮，而大黄素，查特丁和酯4-乙氧基苯甲酸是最主要的成分。通过微稀释分析获得的结果表明，FA具有很强的抗菌活性。此外，生物膜生物质的结晶紫染色表明，提取物对所有测试菌株的生物膜形成均具有很强的影响，而对预先形成的生物膜的影响则不太明显。通过观察到生物膜结构变化的扫描电子显微镜证实了对生物膜的影响。提取物对O ₂消耗和CO产生的活性使用Micro-Oxymax呼吸计监测₂。有趣的是，浮游形式以及生物膜中大多数菌株的呼吸作用均降低。这项研究获得的结果为进一步研究FA提取物对生物膜与呼吸之间的联系起作用的机制提供了良好的基础。