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MaPmt1, a protein O-mannosyltransferase, contributes to virulence through governing the appressorium turgor pressure in Metarhizium acridum
Fungal Genetics and Biology ( IF 3 ) Pub Date : 2020-10-29 , DOI: 10.1016/j.fgb.2020.103480
Zhiqiong Wen 1 , Huiting Tian 1 , Yuxian Xia 1 , Kai Jin 1
Affiliation  

O-glycosylation is a very important post-translational modification of protein and involved in many cell processes in fungi. There exist three protein O-manosyltransferanse genes (MaPmt1, MaPmt2, MaPmt4) in Metarhizium acridum based on sequence homology. Here, MaPmt1, a gene for Pmt1 O-manosyltransferanse in M. acridum, was characterized and functionally analyzed through targeted gene disruption and complementation methods. Deletion of MaPmt1 had no effect on conidial germination, but slightly increased the conidial yield and significantly impaired fungal tolerances to UV-B radiation and wet-heat. Deletion of MaPmt1 made the fungus become more sensitive to cell wall disturbing agents and exhibit a thinner cell wall with changed components. Insect bioassays showed that disruption of MaPmt1 attenuated the fungal virulence significantly by topical inoculation but not by injection, indicating that MaPmt1 is required for penetration during the infection of M. acridum. Interestingly, deletion of MaPmt1 did not affect appressorium formation but significantly decreased appressorium turgor pressure. Moreover, the decreased virulence of MaPmt1 disruptant is mainly due to the reduced appressorium turgor pressure, which may be resulted from the declined glycerol concentration, combined with the weakened cell wall that could not hold the normal appressorium turgor pressure to penetrate the host cuticle.



中文翻译:

MaPmt1 是一种蛋白质 O-甘露糖基转移酶,通过控制绿僵菌中的附着壁膨压来促进毒力

O-糖基化是一种非常重要的蛋白质翻译后修饰,参与真菌的许多细胞过程。基于序列同源性,绿僵菌中存在三个蛋白O-甘露糖基转移酶基因(MaPmt1MaPmt2MaPmt4)。这里,MaPmt1,用于PMT1基因ö在-manosyltransferanse M. acridum,进行了表征,并通过定向基因破坏和互补的方法在功能上进行分析。删除MaPmt1对孢子萌发没有影响,但略微增加了孢子产量,并显着削弱了真菌对 UV-B 辐射和湿热的耐受性。MaPmt1 的缺失使真菌对细胞壁干扰剂变得更加敏感,并表现出更薄的细胞壁和改变的成分。昆虫生物测定表明,MaPmt1 的破坏通过局部接种显着减弱了真菌毒力,而不是通过注射,表明MaPmt1M. acridum感染过程中是渗透所必需的。有趣的是,MaPmt1 的缺失并不影响附着物的形成,但会显着降低附着物的膨压。此外,毒力减弱MaPmt1 破坏剂主要是由于附着物膨压降低,这可能是由于甘油浓度下降,再加上细胞壁减弱,无法保持正常的附着物膨压以穿透宿主角质层。

更新日期:2020-11-03
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