当前位置: X-MOL 学术Enzyme Microb. Technol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Immobilization of phospholipase D on macroporous SiO2/cationic polymer nano-composited support for the highly efficient synthesis of phosphatidylserine
Enzyme and Microbial Technology ( IF 3.4 ) Pub Date : 2020-12-01 , DOI: 10.1016/j.enzmictec.2020.109696
Jia-Qin Wu 1 , Xiao-Mei Xu 1 , Ding-Lin Wang 1 , Neng-Bing Long 1 , Rui-Feng Zhang 1
Affiliation  

Novel nano-composites were prepared by coating epoxy resin-based cationic polymer in nano-thickness via in-situ curing on the nano-wall of macroporous SiO2 with pore size of 0.5∼1 μm. By changing the thickness of polymer coating the specific surface area and porosity varied in range of 115∼74 m2/g and 90.4∼83.9 %, respectively. Through ion exchange phospholipase D (PLD, from Streptomyces sp) was efficiently immobilized on the nano-composites as support and the immobilized PLD was applied for the highly efficient synthesis of phosphatidylserine (PS). The loading amount of PLD on the nano-composited support reached to a maximum of 90.2 mg/gsupport, 4 times as high as that on the pure macroporous silica. The specific activity of the immobilized PLD reached as high as 16,230 U/gprotein, while that of free PLD was 18,780 U/gprotein. Under a wide range of temperature and pH the stability and activity of the immobilized PLD were greatly improved as compared with the free ones. Under optimized conditions at 45 °C and pH 7.0, the PS yield reached as high as 96.2 % within 40 min. After 28 days storage the immobilized PLD retained 82.2 % of original activity, and after 12 cycling reuses it retained 79.3 % of PS yield, which indicated that the immobilized PLD exhibited good stability.

中文翻译:

将磷脂酶 D 固定在大孔 SiO2/阳离子聚合物纳米复合载体上以高效合成磷脂酰丝氨酸

通过在孔径为0.5~1 μm的大孔SiO2纳米壁上原位固化涂覆环氧树脂基阳离子聚合物,制备了新型纳米复合材料。通过改变聚合物涂层的厚度,比表面积和孔隙率分别在 115~74 m2/g 和 90.4~83.9% 的范围内变化。通过离子交换磷脂酶 D (PLD, from Streptomyces sp) 被有效地固定在作为载体的纳米复合材料上, 固定化的 PLD 被用于高效合成磷脂酰丝氨酸 (PS)。PLD在纳米复合载体上的负载量最高可达90.2 mg/g载体,是纯大孔二氧化硅负载量的4倍。固定化PLD的比活性高达16,230 U/gprotein,而游离PLD的比活性高达18,780 U/gprotein。在较宽的温度和pH范围内,固定化PLD的稳定性和活性与游离PLD相比有很大提高。在 45 °C 和 pH 7.0 的优化条件下,PS 产率在 40 分钟内高达 96.2%。储存28天后,固定化PLD保留了82.2%的原始活性,12次循环重复使用后保留了79.3%的PS收率,表明固定化PLD表现出良好的稳定性。
更新日期:2020-12-01
down
wechat
bug