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Co-exposure of iron oxide nanoparticles and glyphosate-based herbicide induces DNA damage and mutagenic effects in the guppy (Poecilia reticulata)
Environmental Toxicology and Pharmacology ( IF 4.3 ) Pub Date : 2020-10-23 , DOI: 10.1016/j.etap.2020.103521
Nicholas Silvestre de Souza Trigueiro , Bruno Bastos Gonçalves , Felipe Cirqueira Dias , Emília Celma de Oliveira Lima , Thiago Lopes Rocha , Simone Maria Teixeira Sabóia-Morais

Iron oxide nanoparticles (IONPs) have been tested to remediate aquatic environments polluted by chemicals, such as pesticides. However, their interactive effects on aquatic organisms remain unknown. This study aimed to investigate the genotoxicity and mutagenicity of co-exposure of IONPs (γ-Fe2O3 NPs) and glyphosate-based herbicide (GBH) in the fish Poecilia reticulata. Thus, fish were exposed to citrate-functionalized γ-Fe2O3 NPs (0.3 mg L−1; 5.44 nm) alone or co-exposed to γ-Fe2O3 NPs (0.3 mg L−1) and GBH (65 and 130 μg of glyphosate L−1) during 14 and 21 days. The genotoxicity (DNA damage) was analyzed by comet assay, while the mutagenicity evaluated by micronucleus test (MN test) and erythrocyte nuclear abnormalities (ENA) frequency. The co-exposure induced clastogenic (DNA damage) and aneugenic (nuclear alterations) effects on guppies in a time-dependent pattern. Fish co-exposed to NPs and GBH (130 μg glyphosate L−1) showed high DNA damage when compared to NPs alone and control group, indicating synergic effects after 21 days of exposure. However, mutagenic effects (ENA) were observed in the exposure groups after 14 and 21 days. Results showed the potential genotoxic and mutagenic effects of maghemite NPs and GBH co-exposure to freshwater fish. The transformation and interaction of iron oxide nanoparticles with other pollutants, as herbicides, in the aquatic systems are critical factors in the environmental risk assessment of metal-based NPs.



中文翻译:

氧化铁纳米颗粒和草甘膦基除草剂的共同暴露诱导孔雀鱼(Poecilia reticulata)的DNA损伤和诱变作用

已经测试了氧化铁纳米粒子(IONPs)来补救被化学物质(例如农药)污染的水生环境。然而,它们对水生生物的交互作用仍然未知。本研究旨在探讨IONPs(γ-铁的同时暴露的遗传毒性和致突变性2个Ø 3在鱼NPS)和基于草甘膦除草剂(GBH)孔雀鱼。因此,鱼暴露于柠檬酸官能化的γ-的Fe 2个ö 3纳米颗粒(0.3毫克的L -1 ; 5.44纳米)单独或共同暴露于γ-的Fe 2 ö 3纳米颗粒(0.3毫克的L -1)和GBH(65和130μg草甘膦L -1)在14天和21天之内。遗传毒性(DNA损伤)通过彗星分析进行了分析,而诱变性则通过微核试验(MN试验)和红细胞核异常(ENA)频率进行了评估。共同暴露对孔雀鱼产生的消灭性(DNA损伤)和非致癌性(核改变)的影响呈时间依赖性。鱼与NP和GBH共同暴露(130μg草甘膦L -1)与单独的NP和对照组相比,显示出高的DNA损伤,表明暴露21天后具有协同作用。但是,暴露组在14天和21天后观察到了致突变作用(ENA)。结果表明,磁赤铁矿和GBH共同暴露于淡水鱼具有潜在的遗传毒性和诱变作用。氧化铁纳米颗粒与其他污染物(例如除草剂)在水生系统中的转化和相互作用是评估金属基NP的环境风险的关键因素。

更新日期:2020-10-30
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