Abstract Dusky cotton bug, Oxycarenus hyalinipennis Costa (Hemiptera: Lygaeidae) is a major pest of cotton crop across various regions in the world, and has developed resistance to a number of insecticides including bifenthrin. In the current study, we evaluated the genetics and realized heritability (h2) of bifenthrin resistance following selection of O. hyalinipennis in the laboratory for 15 generations. The selected population of O. hyalinipennis had a resistance ratio of 554.21-fold as compared to a laboratory susceptible population. Toxicity data of reciprocal crosses F1 and F1’ revealed that bifenthrin resistance was inherited as an autosomal trait and no maternal influence was involved. The degree of dominance (DLC) values, 0.68 and 0.81 for F1 and F1’, respectively, revealed that incompletely dominant genes were governing the bifenthrin resistance in O. hyalinipennis. Resistance to bifenthrin also shifted from completely dominant (DML = 0.95) to fully recessive (DML = 0.08) trait upon changing the concentrations of bifenthrin from 165.25 ppm to 2500 ppm, respectively. The chi-square analysis of monogenic model in both back-crosses, BC1 (F1♀ × S♂) and BC2 (F1♀ × R♂) resulted in rejection of the hypothesis of monogenic resistance, which indicated that multiple factors were involved in controlling bifenthrin resistance. The estimated value of realized heritability (h2) for bifenthrin resistance was 0.09. Selection of O. hyalinipennis with bifenthrin for 15 generations induced a high resistance to bifenthrin that was autosomal, incompletely dominant and polygenic in nature. A low heritability value revealed that bifenthrin resistance could likely develop in the field but at much slower pace. These results have important implications for bifenthrin resistance management in O. hyalinipennis.

中文翻译：

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巴基斯坦暗色棉虫 Oxycarenus hyalinipennis (Costa) (Hemiptera: Lygaeidae) 中选择联苯菊酯抗性的遗传基础表征和实现的遗传力

摘要 暗色棉蝽，Oxycarenus hyalinipennis Costa（半翅目：Lygaeidae）是世界各地棉花作物的主要害虫，对包括联苯菊酯在内的多种杀虫剂产生抗药性。在目前的研究中，我们评估了遗传学并实现了在实验室选择 O. hyalinipennis 15 代后联苯菊酯抗性的遗传力 (h2)。与实验室易感群体相比，选定的 O. hyalinipennis 群体具有 554.21 倍的抗性比。F1 和 F1' 互交的毒性数据显示联苯菊酯抗性作为常染色体性状遗传，不涉及母体影响。F1 和 F1' 的优势度 (DLC) 值分别为 0.68 和 0.81，揭示了不完全显性基因控制着 O. hyalinipennis 的联苯菊酯抗性。当联苯菊酯的浓度分别从 165.25 ppm 变为 2500 ppm 时，对联苯菊酯的抗性也从完全显性 (DML = 0.95) 转变为完全隐性 (DML = 0.08) 性状。BC1(F1♀×S♂)和BC2(F1♀×R♂)两种回交单基因模型的卡方分析均拒绝单基因抗性假设，表明多因素参与控制联苯菊酯抗性。联苯菊酯抗性的已实现遗传力 (h2) 的估计值为 0.09。用联苯菊酯选择 O. hyalinipennis 15 代诱导了对联苯菊酯的高抗性，这种抗性是常染色体、不完全显性和多基因性质的。低遗传力值表明联苯菊酯抗性可能会在田间产生，但速度要慢得多。这些结果对 O. hyalinipennis 的联苯菊酯抗性管理具有重要意义。