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Detection of Bartonella sp. and a novel spotted fever group Rickettsia sp. in Neotropical fleas of wild rodents (Cricetidae) from Southern Brazil
Comparative Immunology, Microbiology and Infectious Diseases ( IF 2 ) Pub Date : 2020-10-22 , DOI: 10.1016/j.cimid.2020.101568
Diogo Schott , Karen Umeno , Bruno Dall’Agnol , Ugo Araújo Souza , Anelise Webster , Thais Michel , Felipe Peters , Alexandre Uarth Christoff , Marcos Rogério André , Ricardo Ott , Márcia Jardim , José Reck

The Neotropical region shows a great diversity of fleas, comprising more than 50 genera. The importance of the study of fleas is linked to their potential role as disease vectors. The aim of this study is to investigate the presence of Rickettsia spp. and Bartonella spp. in Neotropical fleas collected from wild rodents in Southern Brazil. From 350 rodents captured, 30 were parasitized by fleas. A total of 61 fleas belonging to two genera and six different species were collected (Craneopsylla minerva minerva, Polygenis occidentalis occidentalis, Polygenis platensis, Polygenis pradoi, Polygenis rimatus, and Polygenis roberti roberti). In 13 % of fleas of three different species (C. minerva, P. platensis, and P. pradoi) Rickettsia sp. DNA was found. Phylogenetic analysis of concatenated sequences of gltA, htrA, and ompA genes showed that Rickettsia sp. found in rodent fleas (referred as strain Taim) grouped together with Spotted Fever Group Rickettsia. In reference to Bartonella spp., five genotypes were identified in seven fleas of two species (C. minerva and P. platensis) and in five rodent spleens. Also, 207 frozen samples of wild rodents were screened for these pathogens: while none was positive for Rickettsia spp.; five rodent spleens were PCR-positive for Bartonella spp.. Herein, we show the detection of potential novel variants of Bartonella sp. and Rickettsia sp. in fleas collected of wild rodents from Southern Brazil. Further studies are needed to fully characterize these microorganisms, as well as to improve the knowledge on the potential role of Neotropical flea species as diseases vectors.



中文翻译:

Bartonella sp。的检测 和一个新发现的发烧组立克次体sp。巴西南部的野生啮齿动物(C科)的新热带蚤

新热带地区的跳蚤种类繁多,包括50多个属。研究跳蚤的重要性与其作为疾病媒介的潜在作用有关。这项研究的目的是调查立克次体的存在。和Bartonella spp。从巴西南部的野生啮齿动物中采集的新热带跳蚤。从捕获的350只啮齿动物中,有30只被跳蚤寄生。共属于两个属61只跳蚤和六种不同的物种收集(Craneopsylla米纳瓦米纳瓦,Polygenis望江南望江南,Polygenis螺旋藻Polygenis pradoi,Polygenis rimatusPolygenis ROBERTI ROBERTI)。在三种不同物种的跳蚤中,有13%(C.米纳瓦,P.螺旋藻,和P. pradoi立克次体属。发现了DNA。系统发育分析的gltAhtrAompA基因的串联序列显示立克次体。发现于啮齿类动物跳蚤(称为“ Taim菌株”)中,与斑点热病立克次体一起出现。关于Bartonella spp。,在两个物种(C. minervaP. platensis)的七个跳蚤和五个啮齿类动物的脾脏中鉴定出五种基因型。此外,还对207份野生啮齿动物冷冻样品进行了这些病原体筛查:而立克次体无阳性spp .; 五个啮齿动物脾对巴尔通体的PCR呈阳性。在这里,我们显示了对巴尔通体sp的潜在新变异的检测。和立克次氏体属。来自巴西南部的野生啮齿动物的跳蚤。需要进一步研究以充分表征这些微生物,并提高对新热带跳蚤物种作为疾病载体的潜在作用的认识。

更新日期:2020-10-30
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