In order to improve the immunogenicity of peptide-based vaccines against group A Streptococcus (GAS), lipid moieties (C16 lipoamino acid and cholic acid) were conjugated with peptide antigen (P25-J8) and further modified with α-poly(glutamic acid) (α-PGA). Thus, positively charged lipopeptide vaccine candidates LCP-1 (P25-K(J8)-SS-C16-C16) and LCP-2 (P25-K(J8)-SS-K(cholic acid)) were synthesized. Negatively charged LCP-3 (P25-K(PGA-J8)-SS-K(cholic acid)) was also produced by attaching α-PGA to the J8 N-terminus of LCP-2. Polyelectrolyte complex (PEC) nanoparticles were formulated with heparin and/or trimethyl chitosan (TMC) for delivery of the lipopeptide vaccine candidates. The ability of the antigen-loaded nanoparticles to induce humoral immune responses was examined in outbred female Swiss mice following intranasal immunization. The antibodies produced were opsonic against all clinical GAS isolates tested.

为了提高针对A组链球菌（GAS）的基于肽的疫苗的免疫原性，将脂质部分（C16脂氨基酸和胆酸）与肽抗原（P25-J8）偶联，并进一步用α-聚谷氨酸修饰（α-PGA）。因此，合成了带正电荷的脂肽疫苗候选LCP-1（P25-K（J8）-SS-C16-C16）和LCP-2（P25-K（J8）-SS-K（胆酸））。带负电荷的LCP-3（P25-K（PGA-J8）-SS-K（胆酸））也通过将α-PGA连接到J8 N-LCP-2的末端。用肝素和/或三甲基壳聚糖（TMC）配制聚电解质复合物（PEC）纳米颗粒，以输送脂肽疫苗候选物。在鼻内免疫后，在近交雌性Swiss小鼠中检查了载有抗原的纳米颗粒诱导体液免疫反应的能力。产生的抗体对所有测试的临床GAS分离物均具有调理作用。