A valuable plant, Cyclobalanopsis gilva, (C. gilva) has a low germination rate (below 50%) under its natural habitations. In order to examine the reasons for the low germination rate, the seeds of C. gilva (germinated and non-germinated) were evaluated using comparative proteomics analysis. A total of 3078 differentially abundant proteins (DAPs) were identified through a label-free method; most DAPs up-accumulated in germinated seeds were related to carbohydrates metabolism. Furthermore the proteins related to the signals, stress, and protein metabolism showed up-accumulation in germinated and no abundance or down-accumulation in non-germinated seeds. Enzyme activity of HK, PGK, PFK, and PK from glycolysis in SG-Control samples were 1.7-, 1.1-, 1.4-, and 1.3-times higher compared with those in control ones while CS, NAD-MDH, α-KGDH, and ICDH from the TCA cycle in SG-Control samples were 3, 1.1, 1.2, and 1.2 times higher than those in NG-Control ones. The β-amylase activity was 4-fold higher in successfully germinated seeds compared to non-germinated seeds. Interestingly, α-amylase did not show significant changes in protein abundance and enzyme activity among the three samples. The present findings reveal that unsuccessful germination of C. gilva seeds is due to lack of energy.

珍贵的植物Cyclobalanopsis gilva（C. gilva）在其自然栖息地下发芽率较低（低于50％）。为了研究发芽率低的原因，吉尔吉斯种子（比较发芽和未发芽）使用比较蛋白质组学分析进行评估。通过无标记方法鉴定出总共3078个差异丰富的蛋白质（DAP）；发芽种子中积累的大多数DAP与碳水化合物的代谢有关。此外，与信号，胁迫和蛋白质代谢有关的蛋白质在发芽的种子中显示出向上积累，而在未发芽的种子中则没有丰度或下降。SG对照样品中糖酵解的HK，PGK，PFK和PK的酶活性分别比CS，NAD-MDH，α-KGDH，CS，NAD-MDH，α-KGDH的对照高1.7、1.1、1.4和1.3倍。 TC-Control样品中TCA循环的ICDH和ICDH分别比NG-Control样品高3、1.1、1.2和1.2倍。成功发芽的种子的β-淀粉酶活性是未发芽种子的4倍。有趣的是，在这三个样品中，α-淀粉酶的蛋白质丰度和酶活性没有显着变化。目前的发现表明，发芽不成功C. gilva种子是由于缺乏能量。