Background

Organic fluorophores embedded in lipid bilayers can nowadays be described by a multiscale computational approach. Combining different length and time scales, a full characterization of the probe localization and optical properties led to novel insight into the effect of the environments.

Scope of review.

Following an introduction on computational advancements, three relevant probes are reviewed that delineate how a multiscale approach can lead to novel insight into the probes' (non) linear optical properties. Attention is paid to the quality of the theoretical description of the optical techniques.

Major conclusions.

Computation can assess a priori novel probes' optical properties and guide the analysis and interpretation of experimental data in novel studies. The properties can be used to gain information on the phase and condition of the surrounding biological environment.

General significance.

Computation showed that a canonical view on some of the probes should be revisited and adapted.

中文翻译：

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脂质膜对荧光探针光学性能的影响

背景

如今，可以通过多尺度计算方法来描述嵌入脂质双层中的有机荧光团。结合不同的长度和时间尺度，对探针定位和光学特性的全面表征导致对环境影响的新颖见解。

审查范围。

在介绍了计算方面的进展之后，对三个相关的探针进行了概述，这些探针描述了多尺度方法如何导致对探针（非线性）线性光学特性的新颖见解。注意光学技术的理论描述的质量。

主要结论。

计算可以评估先验新颖探针的光学性质，并指导新颖研究中实验数据的分析和解释。这些属性可用于获取有关周围生物环境的阶段和条件的信息。

具有一般意义。

计算表明，应该重新研究和修改一些探针的规范视图。