The BlueNative page (BNGE) gel has been the reference technique for studying the electron transport chain organization since it was established 20 years ago. Although the migration of supercomplexes has been demonstrated being real, there are still several concerns about its ability to reveal genuine interactions between respiratory complexes. Moreover, the use of different solubilization conditions generates conflicting interpretations. Here, we thoroughly compare the impact of different digitonin concentrations on the liquid dispersions' physical properties and correlate with the respiratory complexes' migration pattern and supercomplexes. Our results demonstrate that digitonin concentration generates liquid dispersions with specific size and variability critical to distinguish between a real association of complexes from being trapped in the same micelle.

自20年前成立以来，BlueNative页面（BNGE）凝胶一直是研究电子传输链组织的参考技术。尽管已经证明了超级复合物的迁移是真实的，但仍存在一些担忧，表明其揭示呼吸复合物之间真正相互作用的能力。此外，使用不同的增溶条件会产生相互矛盾的解释。在这里，我们彻底比较了不同浓度的洋地黄皂苷对液体分散体物理性质的影响，并与呼吸复合物的迁移模式和超复合物相关。